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首页> 外文期刊>Russian journal of genetics >Genetic Diversity of Solanum pinnatisectum Dun. and Solanum chacoense Bitt. by Resistance to Potato Virus Y and Results of DNA Analysis
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Genetic Diversity of Solanum pinnatisectum Dun. and Solanum chacoense Bitt. by Resistance to Potato Virus Y and Results of DNA Analysis

机译：Solanum Pinnatisectum Dun的遗传多样性。 和solanum chacoense bitt。 通过抗马铃薯病毒Y和DNA分析的结果

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摘要

The potato Solanum tuberosum L. has the greatest diversity of cultivated and wild relatives, whose genetic nature has been studied insufficiently. The aim of the present study was to examine the resistance to PVY in S. chacoense Bitt. and S. pinnatisectum Dun. and to search for DNA markers linked to resistance genes. For the first time, representative populations of two diploid tuber-bearing Solanum species were evaluated by the resistance to PVY and the presence of DNA markers linked to the Ry (extreme resistance) or Ny (hypersensitivity) genes localized on the long arm of chromosome 9. Nine accessions of S. chacoense and six accessions of S. pinnatisectum represented by 168 and 170 genotypes, respectively, from the VIR collection were assessed by resistance to artificial infection with PVY. The differences in segregation of two wild potato species into phenotypic classes with response to PVY infection have been established. The diversity of visible reactions of S. chacoense plants after PVY infection differs from the uniform type of symptoms observed in S. pinnatisectum. DNA analysis was performed in 170 S. chacoense genotypes and 44 S. pinnatisectum genotypes using the Ry186, S1d11, and CT220 markers linked to genes determining viral resistance in potato varieties, breeding clones, or species of the genus Solanum L. Most genotypes of S. chacoense and S. pinnatisectum have amplified CT220 marker linked to the Nx(phu) gene and the Sw-5 gene in the IvP35 line of the species S. phureja Juz. et Buk. and tomato, respectively. The STS Ry186 marker linked to the Ry(chc) gene in Japanese varieties created on the basis of S. chacoense was identified in a small number of S. chacoense genotypes; however, no association between the DNA marker and resistance phenotype was detected. The CAPS S1d11/AcsI marker, which distinguishes between the PVY resistant and susceptible genotypes of both wild potato species, was developed. The plants of two wild-growing tuber-bearing species of Solanum L. were first examined for the presence of the GBSS gene-specific PCR marker (the gene encoding granules-bound starch synthase). A significant part of the genotypes (15% in S. pinnatisectum and 25% in S. chacoense) did not reveal the genetic marker regulating amylose biosynthesis in the forming starch granules. No significant differences in the ability to form tubers depending on the genotypes with or without GBSS marker, indicating the possible presence of an alternative allelic variant of the GBSSI gene in S. chacoense and S. pinnatisectum species, were detected.
机译：马铃薯Solanum Tuberosum L.具有最大的栽培和野生亲属，其遗传性质已经过得不足。本研究的目的是检查S. Chacoense BITT的抗PVY。和S. pinnatisectum dun。并搜索与抗性基因有关的DNA标志物。首次，通过对PVY的抗性和连接到染色体长臂的抗性（极端抵抗）或纽约（过敏）基因连接的DNA标志物的存在，评估了两个二倍体簇生溶朗物种的代表性群体。 。九种含有S. Chacoense和S的六种含有168和170个基因型的Pinnatectum的六种含义，通过抗PVY对人工感染进行了评估。已经建立了两种野生土豆种与对PVY感染反应表型课程的差异的差异。在PVY感染后S. Chaceense植物的可见反应的多样性不同于S. pinnattentum中观察到的均匀类型的症状。使用Ry186，S1D11和CT220标记在170s的Chacoense基因型和44秒中进行DNA分析。与确定马铃薯品种，繁殖克隆或Solanum L.的种类的植物抗性中的病毒性抗性相关的基因的CT220标记物。 。Chacoense和S. pinnatisectum具有扩增与NX（PHU）基因的CT220标记和IVP35系列S.Phureja Juz的IVP35系列中的SW-5基因。 et buk。和番茄。在少数S. Chaceense基因型中鉴定了与S. Chaceense基于S. Chaceense基因型的日本品种中的RY（CHC）基因相关的STS RY186标记;然而，检测到DNA标记和抗性表型之间的关联。开发了显着的S1D11 / ACSI标记，其区分野生土豆种类的PVY抗性和敏感基因型。首先检查两种野生生长的茄子L的植物含Solanum L的植物。存在GBSS基因特异性PCR标记物（编码颗粒状淀粉合酶的基因）。基因型的重要部分（在Chacoenturecturectum中的15％和25％）未揭示成形淀粉颗粒中的遗传标志物调节直链淀粉生物合成。根据有或没有GBSS标记的基因型形成块茎的能力没有显着差异，表明检测到S. Chacoense和S.Pinnatectum物种中的GBSSI基因的替代等位基因变体的存在。

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