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首页> 外文期刊>Russian journal of genetics >Comparative Analysis of Transgenic Tobacco Plants with Different Heterologic Plant Defensive Genes
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Comparative Analysis of Transgenic Tobacco Plants with Different Heterologic Plant Defensive Genes

机译:不同异质植物防御基因转基因烟草植物的比较分析

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摘要

In order to study the possibility of creating new plant forms resistant to phytopathogens, a collection of transgenic plants of model tobacco culture with new different plant protective genes was obtained by the agrobacterial transformation method. First an addition of a collection with serine proteinase inhibitor BWI-1a (ISP) from buckwheat with fragments of a spidroin gene as putable enhancer by vector constructions different designs was done. Secondly, transgenic plants with an antimicrobial peptide from sinthetic wheat Triticum kiharae and with defensine from Stellaria media. Comparative study of physiological characteristics of transgenic plants in biotests in vivo (with isolated leaves) and in vitro (with well biotests) was carried out. Regardless of the design of the vector construction, the target genes were expressed to a different extent in the tissues of all transgenic plants and their seed and vegetative progenies and gave their tissues antibacterial activity, indicating the synthesis of the functional protein. The introduction to the tobacco tissues of the heterologic plant protective genes of different nature that plants use in different defense mechanisms led to a similar increase in antibacterial activity of the transgenic tobacco tissues.
机译:为了研究创造新植物的可能性抗植物病变,通过农杆菌转化方法获得了具有新的不同植物保护基因的模型烟草培养的转基因植物。首先通过载体构造不同的设计,从荞麦与灌注素基因片段的荞麦添加收集。其次,具有来自Sintthetic Meat Triticum Kiharae的抗微生物肽的转基因植物和来自斯蒂利亚培养基的防御性。进行了体内转基因植物生理特性的对比研究(用分离的叶片)和体外(用良好的Biotest)进行。无论矢量结构的设计如何,在所有转基因植物及其种子和种子和营养后代的组织中表达靶基因的不同程度,并给出了它们的组织抗菌活性,表明官能蛋白的合成。不同性质不同性质的异质植物保护基因的烟草组织的引入,其在不同防御机制中使用的影响导致转基因烟草组织的抗菌活性增加。

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