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首页> 外文期刊>Reproductive toxicology >Transcriptomics analysis of early embryonic stem cell differentiation under osteoblast culture conditions: Applications for detection of developmental toxicity
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Transcriptomics analysis of early embryonic stem cell differentiation under osteoblast culture conditions: Applications for detection of developmental toxicity

机译：早期胚胎干细胞分化在成骨细胞培养条件下的转录组体分析：检测发育毒性的应用

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The mouse embryonic stem cell test (mEST) is a promising in vitro assay for predicting developmental toxicity. In the current study, early differentiation of D3 mouse embryonic stem cells (mESCs) under osteoblast culture conditions and embryotoxicity of cadmium sulfate were examined. D3 mESCs were exposed to cadmium sulfate for 24, 48 or 72 h, and whole genome transcriptional profiles were determined. The results indicate a track of differentiation was identified as mESCs differentiate. Biological processes that were associated with differentiation related genes included embryonic development and, specifically, skeletal system development. Cadmium sulfate inhibited mESC differentiation at all three time points. Functional pathway analysis indicated biological pathways affected included those related to skeletal development, renal and reproductive function. In summary, our results suggest that transcriptional profiles are a sensitive indicator of early mESC differentiation. Transcriptomics may improve the predictivity of the mEST by suggesting possible modes of action for tested chemicals. Published by Elsevier Inc.
机译：小鼠胚胎干细胞试验（Mest）是预测发育毒性的有前途的体外测定。在目前的研究中，研究了D3小鼠胚胎干细胞（MESCS）的早期分化在硫酸镉硫酸镉的骨细胞培养条件下和胚胎毒性下。将D3 MESCS暴露于硫酸镉24,48或72小时，并测定全基因组转录型材。结果表明鉴定了分化的轨迹，因为MESCS分辨率。与分化相关基因相关的生物过程包括胚胎发育，具体而言，骨骼系统的开发。硫酸镉在所有三个时间点都抑制了MESC分化。功能性途径分析表明生物途径受影响包括与骨骼发育，肾和生殖功能有关的那些。总之，我们的结果表明转录型材是早期MESC分化的敏感指标。通过表明可能的测试化学品的可能作用方式，转录组织可以改善最初的预测性。 elsevier公司发布

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