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首页> 外文期刊>Odontology >White-to-opaque switching is involved in the phospholipase B production of Candida dubliniensis on Price's egg yolk agar
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White-to-opaque switching is involved in the phospholipase B production of Candida dubliniensis on Price's egg yolk agar

机译：白对不透明的切换涉及在价格的蛋黄琼脂上的磷脂酶B生产Candida Dubliniensis

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摘要

Measuring the production of Candida dubliniensis (C. dubliniensis) phospholipase B (PLase B) by the Price's method has long been considered to be unattainable because the levels of PLase produced are undetectable. In this study, C. dubliniensis, C. glabrata, C. guilliermondii, C. krusei, C. parapsilosis and C. tropicalis were shown to produce PLase B and form clear white zones around their colonies when peptone, a component of the original Price's egg yolk (OP) agar, is replaced with a yeast nitrogen base (YNB). This new medium is named modified Price's (MP) agar. Based on this finding, we propose a new modified Price's (NMP) agar containing 0.75% peptone and 0.25% YNB, which enabled measurement of PLase B production by C. dubliniensis and C. albicans with results consistent with those obtained for C. albicans grown on OP agar. We strongly believe that the MP and NMP agars are very useful for screening PLase B production by C. dubliniensis and non-albicans Candida spp. Moreover, the addition of several bioactive agents (the proteinase inhibitors pepstatin A and saquinavir, the calcineurin inhibitors cyclosporine A and tacrolimus, the cell-permeable cAMP analog dBcAMP, and the quorum-sensing molecule farnesol) to the OP agar enhanced PLase B production by C. dubliniensis. During the course of our study to clarify the reason why PLase B was not produced, we found that C. dubliniensis cells grown on OP agar undergo a white-to-opaque transition, which may explain why they showed minimal production of PLase B on this medium.
机译：通过价格的方法测量丹丹Dubliniensis（C. Dubliniensis）磷脂酶B（Plase B）的生产长期以来一直被认为是无法实现的，因为所产生的素质水平是不可检测的。在本研究中，C. Dubliniensis，C.Glabrata，C.Guilliermondii，C.Krusei，C.Parapsilosis和C.Tropicalis显示出在蛋白胨，原价的组成部分时形成圆形斑块的透明白色区域蛋黄（OP）琼脂，用酵母氮气碱（YNB）。这个新媒体被命名为修改价格（MP）agar。基于该发现，我们提出了一种含有0.75％蛋白胨和0.25％YNB的新型改性价格（NMP）琼脂，其通过C. Dubliniensis和C.醛型人的蛋白B生产的测量能够与所获得的C. albicans种群的结果一致在op agar。我们强烈地认为，MP和NMP痛苦对于通过C. Dubliniensis和非蛋白质人念珠菌SPP筛选蛋白B生产非常有用。此外，添加几种生物活性剂（蛋白酶抑制剂胃蛋白酶A和Saquinavir，钙酰胺抑制剂环孢菌素A和Tacrolimus，细胞渗透性阵列模拟Dbcamp和仲裁分子法胆汁）通过C. Dubliniensis。在我们的研究过程中，澄清了不产生的原因，我们发现在OP琼脂上生长的C. Dubliniensis细胞经历了白色到不透明的转换，这可以解释为什么它们在此上显示出最小的Plase B生产中等的。

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