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首页> 外文期刊>Russian Journal of Plant Physiology >The Technique of Water Extract Preparation from Goat Willow Leaves with Allowance for Circadian Rhythm of Their Biological Activity to Stimulate Scots Pine Seed Germination
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The Technique of Water Extract Preparation from Goat Willow Leaves with Allowance for Circadian Rhythm of Their Biological Activity to Stimulate Scots Pine Seed Germination

机译：从山羊柳树的水提取物制备技术，允许昼夜节律的生物活性促进苏格兰松树种子萌发

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摘要

The preparative technique for water extraction of goat willow (Salix caprea L.) growing leaves was elaborated in order to stimulate Scots pine (Pinus sylvestris L.) seed germination by the extracts (patent no. 2662999). It was shown that the effects of the extract were accounted for by its biological activity, which considerably varied depending on the time of day for extract sampling. The maximal increase in gibberellin-like activity (equivalent to 3.4-3.6 mu M GA(3)) and marked decrease in ABA-like activity (equivalent to 0.15-0.30 mu M ABA) were recorded in the willow leaf extracts harvested in the morning (8:00 a.m.) or evening (8:00 p.m.) in South Karelia (60 degrees N) in mid-May. Application of the extracts to pine seeds afforded the maximal effects on their germination. Thus, the second-class seeds displayed qualitative characteristics of the first-class seeds (germination exceeding 80%) after the treatment. It is concluded that the introduction of the method into practice will allow utilization of the accessible, widespread plant raw material for production of the effective natural preparation, which is capable of improving sowing qualities of Scots pine seeds.
机译：阐述了山羊柳（Salix Caprea L.）生长叶片水提取的制备方法，以刺激提取物的苏格兰松（Pinus Sylvestris L.）种子萌发（专利No.2662999）。结果表明，提取物的效果被其生物活性占，这取决于提取物抽样的时间大幅度变化。在早晨收获的柳树叶提取物中记录了嗜酸甘油蛋白样活性的最大增加（相当于3.4-3.6μmGa（3））并标记为aba样活性（相当于0.15-0.30μmba） （上午8:00）或晚上（下午8:00）在南卡雷利亚（60摄氏度）。提取物在松籽中的应用得到了对其发芽的最大效应。因此，在治疗后，二等种子显示了一流的种子的定性特征（萌发超过80％）。结论是，将该方法引入实践将允许利用可接近的广泛植物原料，以生产有效的天然制剂，能够改善苏格兰杉木种子的播种品质。

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