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首页> 外文期刊>Oncology letters >Combination of four gene markers to detect circulating tumor cells in the peripheral blood of patients with advanced lung adenocarcinoma using real-time PCR
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Combination of four gene markers to detect circulating tumor cells in the peripheral blood of patients with advanced lung adenocarcinoma using real-time PCR

机译：使用实时PCR检测肺腺癌患者外周血循环肿瘤细胞的四个基因标记物的组合

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摘要

The aim of this study was to establish a robust and reliable assay for the detection of circulating tumor cells (CTCs) in the peripheral blood (PB) of patients with advanced lung adenocarcinoma. We used real-time reverse transcription PCR (RT-PCR) to detect survivin, human telomerase reverse transcriptase (hTERT), cytokeratin-7 (CK-7) and thyroid transcription factor 1 (TTF-1) mRNA expression levels in 68 advanced lung adenocarcinoma patients and 30 healthy patients. Statistical analyses were additionally performed to examine the correlation between the mRNA expression levels of these markers with the clinicopathological features of advanced lung adenocarcinoma patients. The sensitivity of these four mRNA markers in the PB of advanced lung adenocarcinoma patients was 41.18, 61.76, 41.18 and 35.29%, respectively. The sensitivity of these four markers combined was 82.35%, which was significantly higher compared with single marker detection. Statistical analysis demonstrated that high expression levels of survivin, hTERT and TTF-1 mRNA are positively correlated with lymph node classification, and high expression levels of survivin, hTERT, CK7 and TTF-1 mRNA are positively correlated with distant metastasis (P<0.05). In addition, overexpression of these four mRNA markers is positively correlated with disease progression (P<0.05). Our data suggest that the combination of survivin, hTERT, CK-7 and TTF-1 mRNA markers may provide a valuable tool for CTC detection and is associated with disease progression in advanced lung adenocarcinoma patients.
机译：本研究的目的是为检测晚期肺腺癌患者的外周血（PB）中的循环肿瘤细胞（CTC）建立稳健且可靠的测定。我们使用实时逆转录PCR（RT-PCR）来检测Survivin，人端粒酶逆转录酶（HTERT），细胞角蛋白-7（CK-7）和甲状腺转录因子1（TTF-1）mRNA表达水平在68晚期肺部腺癌患者和30名健康患者。另外进行统计分析以检查这些标志物的mRNA表达水平与晚期肺腺癌患者的临床病理特征之间的相关性。本次晚期肺腺癌患者PB中这四种mRNA标志物的敏感性分别为41.18,61.76,41.18和35.29％。与单次标记检测相比，这四个标记的敏感性为82.35％，显着高得多。统计学分析证明，Survivin的高表达水平，Hett和TTF-1 mRNA与淋巴结分类呈正相关，并且Survivin，HTERT，CK7和TTF-1 mRNA的高表达水平与远处转移呈正相关（P <0.05） 。此外，这四个mRNA标记的过表达与疾病进展呈正相关（P <0.05）。我们的数据表明，Survivin，HTERT，CK-7和TTF-1 mRNA标记的组合可以为CTC检测提供有价值的工具，并且与晚期肺腺癌患者的疾病进展相关。

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  • 来源
    《Oncology letters》 |2013年第4期|共7页
  • 作者

    Yu Yan; Xu Gang; Cao Jingyan; Jin Shi; Man Yingchun; Shang Lihua;

  • 作者单位

    Harbin Med Univ Affiliated Hosp 3 Dept Med Oncol Harbin 150080 Peoples R China;

    Harbin Med Univ Affiliated Hosp 3 Dept Med Oncol Harbin 150080 Peoples R China;

    Harbin Med Univ Affiliated Hosp 3 Dept Med Oncol Harbin 150080 Peoples R China;

    Harbin Med Univ Affiliated Hosp 3 Dept Med Oncol Harbin 150080 Peoples R China;

    Harbin Med Univ Affiliated Hosp 3 Dept Med Oncol Harbin 150080 Peoples R China;

    Harbin Med Univ Affiliated Hosp 3 Dept Med Oncol Harbin 150080 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 肿瘤学;
  • 关键词

    circulating tumor cells; quantitative real-time PCR; survivin; hTERT; CK-7; TTF-1;

    机译：循环肿瘤细胞;定量实时PCR;Survivin;HTERT;CK-7;TTF-1;

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