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首页> 外文期刊>Oncology Research >miR-218 Inhibits Proliferation, Migration, and EMT of Gastric Cancer Cells by Targeting WASF3
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miR-218 Inhibits Proliferation, Migration, and EMT of Gastric Cancer Cells by Targeting WASF3

机译：miR-218通过靶向vAsf3来抑制胃癌细胞的增殖，迁移和EMT

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MicroRNAs (miRNAs) play an important role in carcinogenesis. miR-218 is one of the most known miRNAs and has been demonstrated to inhibit progression in gastric cancer. However, the underlying molecular mechanism is not established. In this study, qRT-PCR and Western blot indicated that miR-218 was downregulated in gastric cancer cell lines SGC7901 and BGC823 compared to that in normal gastric epithelial cell line GES-1. MTT and wound scratch assays suggested that overexpression of miR-218 markedly suppressed cell proliferation, migration, and EMT of gastric cancer cells. Furthermore, we proved that WASF3 was a direct target of miR-218 by luciferase reporter assay, and restoration of WASF3 expression impairs miR-218-induced inhibition of proliferation, migration, and EMT in gastric cancer cells SGC7901. In summary, our results demonstrated that miR-218 functions as one of the tumor-suppressive miRNAs and inhibits gastric cancer tumorigenesis by targeting WASF3. miR-218 may serve as a potential therapeutic target for the treatment of gastric cancer.
机译：MicroRNA（miRNA）在致癌中发挥着重要作用。 miR-218是最着名的miRNA之一，已经证明抑制胃癌的进展。但是，底层分子机制未建立。在该研究中，QRT-PCR和Western印迹表明，与正常胃上皮细胞系GES-1相比，MIR-218在胃癌细胞系SGC7901和BGC823中下调。 MTT和伤口划痕测定表明MiR-218的过表达明显抑制了胃癌细胞的细胞增殖，迁移和EMT。此外，我们证明了WASF3是MIR-218的直接靶标，通过荧光素酶报告结果测定，并且恢复为胃癌细胞SGC7901中的胃癌细胞中增殖，迁移和EMT的抑制miR-218诱导的增殖，迁移和EMT。总之，我们的结果表明MIR-218作为肿瘤抑制miRNA之一，通过靶向WASF3来抑制胃癌肿瘤瘤。 miR-218可以作为治疗胃癌的潜在治疗靶标。

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