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首页> 外文期刊>Oral diseases >The growth and osteoclastogenic effects of fibroblasts Isolated from keratocystic odontogenic tumor
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The growth and osteoclastogenic effects of fibroblasts Isolated from keratocystic odontogenic tumor

机译：成纤维细胞分离的成纤维细胞异血性肿瘤的生长和骨质细胞源作用

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OBJECTIVES: To Investigate the growth characteristics and effects on osteoclastogenesfs in ffbroblasts isolated from keratocystic odontogenic tumor (K.CQT) fibrous capsule. MATERIALS AND METHODS: Fibroblasts isolated from K.COT fibrous capsule and normal gingival snucosa were cultured in vitro. Their colony-forming units and proltf-erative activity were investigated, and the osteoclastogenic effects were also observed by a co-culture system with osteoclast precursor cell line Raw264.7. The mRNA of several genes related to bone resorption (IL-6, VEGF, CQX-2, and M-CSF) was analyzed by real-time PCR. RESULTS: Keratocystic odontogenic tumor fibroblasts developed fewer CFU and had longer population doubling time than gingival fibroblasts (P < 0.05). In contrast to gingival fibroblasts, KCOT fibroblasts expressed less IL-6, CQX-2, and M-CSF (P < 0.05); however, the Raw264.7 co-cultured with KCOT fibroblasts developed more osteoclast-like cells and expressed higher level of nfatcl than that co-cultured with gingival fibroblasts. Increased COX-2 expression and VEGF expression were detected in KCOT fibroblasts and Raw264.7 co-culture system (P < 0.05).CONCLUSION: Although KCOT fibroblasts showed lower level of cell proliferation than gingival fibrobfasts, higher osteoclastogenic ability was detected when co-cultured with Raw264.7. These results suggest that the cell-cell interaction in the co-culture system, possibly by increasing COX-2 and VEGF expression, may be responsible for the increased osteoclastogenic effects of KCOT fibroblasts.
机译：目的：探讨从角囊囊性牙型肿瘤（K.CQT）纤维状胶囊中分离的FFbroblasts中骨髓细胞的生长特征和影响。材料和方法：在体外培养从K.Cot纤维胶囊中分离的成纤维细胞和正常牙龈梭菌。研究了它们的菌落形成单位和PROLTF-Perative活性，并且还通过综合培养系统与骨酸骨糖前体细胞系Raw264.7进行了疏松骨细胞源效应。通过实时PCR分析与骨吸收（IL-6，VEGF，CQX-2和M-CSF）相关的几种基因的mRNA。结果：角膜凸畸形的牙科肿瘤成纤维细胞较少的CFU，并且种群倍增时间比牙龈成纤维细胞倍增（P <0.05）。与牙龈成纤维细胞相反，KCOT成纤维细胞表达较少的IL-6，CQX-2和M-CSF（P <0.05）;然而，用KCOT成纤维细胞共同培养的RAW264.7产生更具骨骨细胞样细胞并表达比与牙龈成纤维细胞共培养的更高水平的NFATCL。在KCOT成纤维细胞和Raw264.7共培养系统中检测到增加的COX-2表达和VEGF表达（P <0.05）。结论：虽然KCOT成纤维细胞显示出比牙龈纤维纤维面积的细胞增殖水平较低，但是当共同用Raw264.7培养。这些结果表明，通过增加COX-2和VEGF表达可能是共培养系统中的细胞 - 细胞相互作用可能是kcot成纤维细胞的增加的骨质细胞源性效应。

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