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Identification of Candida species in the clinical laboratory: A review of conventional, commercial, and molecular techniques

机译：临床实验室念珠菌种类的鉴定 - 综合，商业和分子技术综述

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摘要

In healthy individuals, Candida species are considered commensal yeasts of the oral cavity. However, these microorganisms can also act as opportunist pathogens, particularly the so-called non-albicans Candida species that are increasingly recognized as important agents of human infection. Several surveys have documented increased rates of C. glabrata, C. tropicalis, C. guilliermondii, C. dubliniensis, C. parapsilosis, and C. krusei in local and systemic fungal infections. Some of these species are resistant to antifungal agents. Consequently, rapid and correct identification of species can play an important role in the management of candidiasis. Conventional methods for identification of Candida species are based on morphological and physiological attributes. However, accurate identification of all isolates from clinical samples is often complex and time-consuming. Hence, several manual and automated rapid commercial systems for identifying these organisms have been developed, some of which may have significant sensitivity issues. To overcome these limitations, newer molecular typing techniques have been developed that allow accurate and rapid identification of Candida species. This study reviewed the current state of identification methods for yeasts, particularly Candida species.
机译：在健康的个体中，念珠菌物种被认为是口腔的非负面酵母。然而，这些微生物也可以充当机会主义病原体，特别是所谓的非蛋白酶念珠菌物种，越来越被认为是人类感染的重要药物。几种调查记录了C.Grobrata，C.Tropicalis，C. Guilliermondii，C. Dubliniensis，C.Parapsilosis和局部真菌感染的C. Dubliniensis。这些物种中的一些是抗性药物的抗性。因此，物种的快速和正确鉴定可以在念珠菌病的管理中发挥重要作用。鉴定念珠菌物种的常规方法是基于形态学和生理学属性。然而，精确鉴定来自临床样品的所有分离物通常复杂且耗时。因此，已经开发了用于识别这些生物的几种手动和自动化的快速商业系统，其中一些可能具有显着的敏感性问题。为了克服这些限制，已经开发了更新的分子键入技术，其允许准确且快速鉴定念珠菌物种。本研究审查了酵母，特别是念珠菌物种的当前鉴定方法状态。

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  • 来源
    《Oral diseases》 |2014年第4期|共16页
  • 作者

    NeppelenbroekK.H.; SeóR.S.; UrbanV.M.; SilvaS.; DovigoL.N.; JorgeJ.H.; CampanhaN.H.;

  • 作者单位

    Department of Prosthodontics Bauru Dental School University of S?o Paulo-USP Bauru S?o Paulo;

    Department of Prosthodontics Varzea Grande School of Dentistry Varzea Grande Mato Grosso Brazil;

    Department of Dentistry Ponta Grossa State University-UEPG Ponta Grossa Paraná Brazil;

    Department of Biological Engineering Institute for Biotechnology and Bioengineering University of;

    Department of Social Dentistry Araraquara Dental School UNESP - Univ. Estadual Paulista;

    Department of Dental Materials and Prosthodontics Araraquara Dental School UNESP - Univ. Estadual;

    Department of Dentistry Ponta Grossa State University-UEPG Ponta Grossa Paraná Brazil;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 口腔科学;
  • 关键词

    Candida; Candida albicans; Candida spp; Candidiasis; oral; Pathology; oral;

    机译：念珠菌;念珠菌念珠菌;Candida SPP;念珠菌病;口腔;病理学;口头;

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