...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Oral diseases >Mouse ES cells have a potential to differentiate into odontoblast-like cells using hanging drop method
【24h】

Mouse ES cells have a potential to differentiate into odontoblast-like cells using hanging drop method

机译：小鼠ES细胞使用悬挂方法具有区分成像料状细胞的含量

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objectives: We examined whether mouse embryonic stem (ES) cells can differentiate into odontoblast-like cells without epithelial-mesenchymal interaction. Materials and methods: Cells were cultured by the 'hanging drop' method using a collagen type-I scaffold (CS) combined with bone morphogenetic protein (BMP)-4 (CS/BMP-4). Expression of odontoblast-related mRNA and protein, and cell proliferation were performed by reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescence staining and WST-1 assay, respectively. Results: Cells potently expressed odontoblast-related cell marker mRNAs following induction of odontoblastic differentiation. Dentin sialophosphoprotein, a marker of mature odontoblasts, was strongly expressed in differentiated ES cells. The cells also acquired an odontoblast-like functional phenotype, as evidenced by the appearance of alkaline phosphatase activity and calcification. The cell-surface expression of α2, α6, αV and αVβ3 integrin proteins was rapidly upregulated in differentiated cells. Finally, anti-α2 integrin antibody suppressed the expression of odontoblastic markers in cells grown using this culture system, suggesting that α2 integrin expression in ES cells triggers their differentiation into odontoblast-like cells. Conclusions: Mouse ES cells cultured by the 'hanging drop' method are able to differentiate into cells with odontoblast-specific physiological functions and cell-surface integrin protein expression.
机译：目的：我们检查了小鼠胚胎茎（ES）细胞是否可以分化成没有上皮 - 间充质相互作用的异托细胞样细胞。材料和方法：使用胶原型-I支架（Cs）与骨形态发生蛋白（BMP）-4（CS / BMP-4）组合的“悬挂液滴”方法培养细胞。通过逆转录聚合酶链反应（RT-PCR），免疫荧光染色和WST-1测定，对表达异形细胞相关mRNA和蛋白质和细胞增殖。结果：细胞在诱导Odontobolas弹性分化后，细胞有效地表达了与相关的细胞标记mRNA。牙本质唾液酸磷蛋白是成熟的牙卵细胞的标志物，在分化的ES细胞中强烈表达。细胞还获得了异藤细胞样功能表型，如碱性磷酸酶活性和钙化的出现所证明。在分化细胞中快速上调α2，α6，αv和αvβ3整联蛋白蛋白的细胞表面表达。最后，抗α2整联蛋白抗体抑制了使用该培养系统生长的细胞中Odontobolas弹性标记的表达，表明ES细胞中的α2整联蛋白表达触发其分化成异形细胞样细胞。结论：通过“悬挂液滴”方法培养的小鼠ES细胞能够分化成具有特异性细胞特异性生理功能和细胞表面整联蛋白表达的细胞。

著录项

  • 来源
    《Oral diseases》 |2014年第4期|共9页
  • 作者

    KawaiR.; OzekiN.; YamaguchiH.; TanakaT.; NakataK.; MogiM.; NakamuraH.;

  • 作者单位

    Department of Endodontics School of Dentistry Aichi Gakuin University Nagoya Aichi Japan;

    Department of Endodontics School of Dentistry Aichi Gakuin University Nagoya Aichi Japan;

    Department of Endodontics School of Dentistry Aichi Gakuin University Nagoya Aichi Japan;

    Department of Endodontics School of Dentistry Aichi Gakuin University Nagoya Aichi Japan;

    Department of Endodontics School of Dentistry Aichi Gakuin University Nagoya Aichi Japan;

    Department of Medicinal Biochemistry School of Pharmacy Aichi Gakuin University Nagoya Japan;

    Department of Endodontics School of Dentistry Aichi Gakuin University Nagoya Aichi Japan;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 口腔科学;
  • 关键词

    Cell differentiation; Cell-matrix interactions; Collagen; Integrin; Odontoblast; Stem cell;

    机译：细胞分化;细胞 - 基质相互作用;胶原;整合蛋白;Odontoblast;干细胞;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号