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首页> 外文期刊>Oral diseases >Image cytometry DNA DNA ploidy analysis: Correlation between two semi‐automated methods
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Image cytometry DNA DNA ploidy analysis: Correlation between two semi‐automated methods

机译：图像细胞测定DNA DNA倍率分析：两个半自动方法之间的相关性

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Abstract Background and Objectives Quantitation of cell DNA content, DNA ploidy, has been established as a research and prognostic technique for decades. A variety of instruments have been used although only a few commercially available systems have established quality assurance and published outcome data. The aim of this study was to compare two automated systems. Methods Nuclear monolayers were obtained from 112 oral biopsies by enzyme digestion and Feulgen staining. These were scanned on both the Fairfield and the Ploidy Work Station ( PWS ) systems. The overall ploidy diagnosis, number of epithelial nuclei, coefficient of variation ( CV ) and 5c exceeding rate (5 CER ) were compared by quantile–quantile plots, t test, Wilcoxon and Spearman's tests. Results The PWS system identified more nuclei ( p ??0.0001) at a lower CV ( p ??0.0001). Using the PWS system, fewer samples were classified as indeterminate. No difference between 5 CER was found between systems ( p ??0.54). There was complete concordance between the two systems in terms of DNA ploidy diagnosis. Conclusions The PWS system is comparable to the Fairfield system for determination of DNA ploidy and has advantages that may lead to improved performance.
机译：摘要背景和目标细胞DNA含量的定量DNA倍增性，已成为几十年的研究和预后技术。已经使用了各种仪器，尽管只有少数商业上可获得的系统已经建立了质量保证和已发表的结果数据。本研究的目的是比较两个自动化系统。方法通过酶消化和Feulgen染色从112个口腔活组织检查获得核单层。这些被扫描在Fairfield和倍性工作站（PWS）系统上。通过定量定数曲线，T检验，威尔科克森和Spearman的测试，比较总倍率诊断，上皮核，变异系数和5C超过速率（5C）进行比较。结果PWS系统在较低的CV（p≤≤0101）下鉴定更多的核（p≤≤0.0001）。使用PWS系统，将样品较少分类为不确定。在系统之间发现了5个CER之间的差异（p？＆ 0.54）。在DNA倍率诊断方面，两种系统之间存在完全一致。结论PWS系统可与用于测定DNA倍性的Fairfield系统相媲美，具有可能导致改进性能的优点。

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