...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Oral diseases >Oral microbiomes in children with asthma and dental caries
【24h】

Oral microbiomes in children with asthma and dental caries

机译：哮喘和龋齿的儿童口腔微生物

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract Objective Recently, a significant association between dental caries and the severity of bronchial asthma in children has been revealed. This finding indicates a possible relationship between the oral microbiome and the pathogenesis of asthma. The purpose of our study was to estimate differences in the dental plaque microbiota of asthmatic children with and without dental caries by 16S rDNA sequencing. Material and methods Dental plaque samples were obtained with a spoon excavator from the occlusal surface of one deciduous tooth (the second mandibular left molar in caries‐free children and the most affected tooth in caries‐affected children). Total DNA was extracted from dental plaque. DNA libraries were analysed by 16S rRNA gene sequencing on the MiSeq (Illumina) platform. Results There were no significant differences in the composition of bacterial communities from both caries‐affected and caries‐free children with asthma. The “caries‐enriched” genus was Veillonella (Veillonellaceae, Selenomonadales, Negativicutes). Relative abundance of Neisseria was significantly higher in caries‐free children with asthma ( p ??0.05). Conclusions The most significant difference in compared bacterial communities was a higher relative abundance of Veillonella in caries‐affected plaques that suggests its involvement in pathogenesis of caries. Potential respiratory pathogens are present in oral cavity of both caries‐affected and caries‐free asthmatic children.
机译：摘要目的是揭示了龋齿与儿童支气管哮喘严重程度之间的重大关联。该发现表明口腔微生物组和哮喘发病机制之间的可能关系。我们研究的目的是估算哮喘患儿的牙菌斑微生物菌，患有16S rdNA测序的牙科龋病的牙菌斑微生物群。材料和方法用勺子挖掘机从一个落叶牙齿的胶囊表面（龋齿儿童的第二个下颌左臼齿和受龋病的儿童中最受影响的牙齿）获得牙科斑块样品。从牙菌斑中提取总DNA。通过16S rRNA基因测序在Miseq（Illumina）平台上进行分析DNA文库。结果患有龋病和无龋儿的细菌群体组成无显着差异。 “富龋”属是veillonella（Veillonellaceae，Selenomona大致，否定）。在没有哮喘的无龋病儿童（P≥β0）中，龋病的相对丰度显着高。结论比较细菌社区的最重要差异是龋齿影响斑块的高度丰度，这表明其参与龋病发病机制。潜在的呼吸道病原体存在于受龋病和无龋的哮喘儿童的口腔中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号