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首页> 外文期刊>Scientia horticulturae >Dynamic changes of miR166s at both the transcriptional and post-transcriptional levels during somatic embryogenesis in Lilium
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Dynamic changes of miR166s at both the transcriptional and post-transcriptional levels during somatic embryogenesis in Lilium

机译：百年体细胞胚胎发生过程中转录和转录后水平的MiR166s的动态变化

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摘要

miR166s are involved in fundamentally important biological processes in plants and exhibit functional redundancy and specificity. However, the regulatory role of miR166-mediated somatic embryogenesis (SE) in plants remains unclear. Our previous results preliminarily identified four miR166 family members that have diverse expression profiles during SE in Lilium pumilum DC. Fisch. and in Lilium davidii var. unicolor. In the present study, phylogenetic inference indicated that pre-miR166s are relatively diverse within a species but are closely related among different Lilium species, suggesting that plant miRNA precursors are complex. The predicted targets of LMBR1, IDD1 and PHB were identified by RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE), and we dynamically monitored miR166s at both the transcriptional and post-transcriptional levels via qRT-PCR. The results indicated that the expression of miR166 precursors and targets was negatively regulated by miR166 s. Moreover, the expression profiles of miR166 family members differed between the two lily species, and the expression levels of miR166 family members also varied among different members of the same species. Lpu-miR166a was expressed at low levels in the embryonic callus and embryo maturation stages, but lpu-miR166b, lda-miR166a and lda-miR166b were expressed at high levels in the torpedo and cotyledon stages. The first gene studied in plant SE, LMBR1, was expressed at high levels during the embryonic callus and late SE stages, suggesting that this gene may be crucial for embryonic cell formation and embryo germination.
机译：MIR166S参与植物中的基本重要的生物过程，表现出功能冗余和特异性。然而，miR166介导的细胞胚胎发生（SE）在植物中的调节作用仍不清楚。我们以前的结果预先确定了在Liliumpumilum DC中具有不同表达型材的MIR166家族成员。菲舍奇。在百合Davidii var。 unicolor。在本研究中，系统发育推理表明，在物种中，MIR166S比例相对不同，但在不同的百合物种中密切相关，表明植物miRNA前体是复杂的。通过RNA连接酶介导的CDNA末端（RLM族）的RNA连接酶介导的预测靶标介绍了CDNA末端（RLM族），并且我们通过QRT-PCR动态监测转录和转录后水平的MIR166s。结果表明MiR166前体和靶标的表达由MiR166 S负调节。此外，MiR166家族成员的表达谱不同于两种百合物种之间，MiR166家族成员的表达水平也在相同物种的不同成员之间变化。 LPU-MIR166A在胚胎呼应和胚胎成熟阶段的低水平表达，但LPU-MIR166B，LDA-MIR166A和LDA-MIR166B在鱼雷和子叶阶段的高水平表达。在植物SE中研究的第一个基因LMBr1在胚胎愈伤组织和晚期阶段的高水平表达，表明该基因对于胚胎细胞形成和胚胎萌发可能是至关重要的。

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