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Practical methods for rapid seed germination from seed coat-imposed dormancy of Prunus yedoensis

机译:从种子涂层纯化的纯化幼苗浸血的实用方法

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The seeds of Prunus yedoensis have slow and poor germination when intact (with endocarp). Cold and/or warm stratification, washing, and scarification with liquid nitrogen and sulfuric acid, in combination with or without gibberellic acid (GA(3)) application, were investigated as facilitators for rapid and high germination. No germination was observed without GA(3) in any treatment for intact seeds. GA(3) treatment for one day significantly shortens the mean germination time (MGT) and improves seed germination percentage. Immersion in liquid nitrogen followed by GA(3) treatment, GA(3) application to intact seeds and seed coat removed seeds significantly enhanced the germination and MGT to 63 +/- 7.6% (8.6 weeks), 71 +/- 5.0% (4.7 weeks), and 93 +/- 5.1% (0.8 weeks), compared to untreated seeds or stratified seeds (0%) chilled for six months. Depending on the GA(3) application method, results could be different in intact seeds. GA(3) application with vigorous shaking accomplished higher germination and lower MGT (71 +/- 5.0%, 4.7 weeks) than gentle shaking incubation (33 +/- 6.0%, 10.4 weeks). The germination inhibitor(s) were located in the seed coat because seed coat extract inhibited germination in decoated seeds. Therefore, coat removal alleviates the inability to promote germination and it has the same beneficial effect as does the application of GA(3) . According to the results, it suggest that the effective application of GA(3) on intact seeds or seed coat removal could overcome seed dormancy in P. yedoensis.
机译:当完整时(完整)(用endocarp)时,养鸡腺的种子具有缓慢和差的萌发。与液氮和硫酸的冷和/或热分层,与硫酸(Ga(3))应用组合的冷和/或热分层,与硫酸(Ga(3))应用,作为快速和高萌发的促进剂。在任何对完整种子的治疗中没有观察到没有Ga(3)的萌发。 Ga(3)治疗一天显着缩短平均发芽时间(MGT)并改善种子萌发百分比。浸入液氮中,然后由Ga(3)处理,Ga(3)施用完整的种子和种子涂层除去种子显着增强了萌发和MgT至63 +/- 7.6%(8.6周),71 +/- 5.0%( 4.7周)和93 +/- 5.1%(0.8周),与未处理的种子或分层种子(0%)冷却六个月。根据GA(3)施用方法,结果可能在完整的种子中不同。 Ga(3)剧烈摇动的应用更高的萌发和低MgT(71 +/- 5.0%,4.7周),而不是温和振动孵化(33 +/- 6.0%,10.4周)。萌发抑制剂位于种子涂层中,因为种子涂层提取物抑制了陶醉的种子中的发芽。因此,涂层去除减轻了无法促进发芽的能力,并且具有与Ga(3)的应用相同的有益效果。根据结果​​,它表明,Ga(3)对完整的种子或种子涂层的有效应用可以克服P. Yedoensis中的种子休眠。

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