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Effects the mechanism of micro-vacuum storage on broccoli chlorophyll degradation and builds prediction model of chlorophyll content based on the color parameter changes

机译:基于颜色参数变化,影响微真空储存对西兰花叶绿素劣化的机理,构建叶绿素含量的预测模型

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The objective of this study was to investigate the mechanism of micro-vacuum (MV) storage on broccoli chlorophyll degradation and builds prediction model of chlorophyll content based on the color parameter changes. The freshly harvested broccoli were subjected to MV storage (70 +/- 5 kPa) and atmospheric pressure storage at 3 +/- 1 degrees C for 49 d; The relationship between broccoli chlorophyll content and its surface color parameters (-a/b value) was studied to build broccoli chlorophyll content prediction model based on the color parameter changes under MV conditions. The results showed the degradation of chlorophyll could be inhibited, activities of CAT, SOD, POD could be improved, superoxide anion (O-2 center dot) release rate, hydrogen peroxide (H2O2) content, malondiadehyde (MDA) content and the increase of membrane permeability could be decreased significantly in MV storage conditions; the correlation analysis showed that relationship between chlorophyll degradation rate and activities of CAT, SOD, POD, the relationship between chlorophyll content and O-2(-)center dot release rate, H2O2 content, MDA content, membrane permeability showed a significant negative correlation; The changes of chloroplast ultrastructure could be inhibited and degradation of chlorophyll could be delayed in MV conditions, yellowing mechanisms of broccoli was revealed by the cellular level in MV conditions. Validation showed that the prediction model could reflect the changes of chlorophyll content during storage, provide theoretical basis for the further application of non-destructive testing technology.
机译:本研究的目的是研究微真空(MV)储存对西兰花叶绿素降解的机制,并基于颜色参数变化构建叶绿素含量的预测模型。将新鲜收获的西兰花在3 +/- 1℃下进行MV储存(70 +/- 5kPa)和大气压储存49d;研究了西兰花叶绿素含量及其表面颜色参数(-A / B值)的关系,基于MV条件下的颜色参数变化构建西兰花叶绿素含量预测模型。结果表明,可以抑制叶绿素的降解,猫,SOD,豆荚的活性,超氧化物阴离子(O-2中心点)释放率,过氧化氢(H2O2)含量,丙二醛(MDA)含量和增加在MV储存条件下可以显着降低膜渗透性;相关分析表明,叶绿素降解速率与猫,SOD,豆荚,叶绿素含量和O-2( - )中心点释放率,H 2 O 2含量,MDA含量,膜渗透性的关系的关系显示出显着的负相关性;可以抑制叶绿体超微结构的变化,并且叶绿素的降解可以延迟MV条件,通过MV条件下的细胞水平揭示了西兰花的黄变理机制。验证表明,预测模型可以反映储存过程中叶绿素含量的变化,为进一步应用非破坏性测试技术提供理论依据。

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