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首页> 外文期刊>Scientia horticulturae >Cold priming the chickpea seeds imparts reproductive cold tolerance by reprogramming the turnover of carbohydrates, osmo-protectants and redox components in leaves
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Cold priming the chickpea seeds imparts reproductive cold tolerance by reprogramming the turnover of carbohydrates, osmo-protectants and redox components in leaves

机译：冷启动鹰嘴豆种子通过重新编程碳水化合物，渗透压保护剂和叶子的氧化还原成分的营业额来赋予生殖抗耐寒性

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摘要

Chickpea, a vital food legume, shows high sensitivity to cold stress at reproductive stage, and temperatures below 20/10 degrees C, (as day/night temperature) at this stage result in disruption in developmental and functional aspects of reproductive components, causing floral abortion, poor pods and significant yield losses. In the present study, chickpea seeds (a cold sensitive genotype GPF2) were cold primed at 5 degrees C +/- 1 degrees C in the dark for 30 days, followed by gradual air-drying at 15 degrees C to the original moisture content (11-13%). The crop was raised in the month of November from non-primed and primed seeds in the outdoor environment (temperature profile: 11.7 degrees C; mean day/night temperature; 1300-1500 mu mol m(-2) s(-1) light intensity, 65-70% relative humidity), until the onset of budding and flowering (45 days after sowing). Subsequently, the plants were separated into different sets to impose following treatments in controlled environment a) control (at optimum temperature; a set of plants were grown at optimum temperature (25/18 degrees C) until maturity, b) cold-stressed; a set of the plants were grown at low temperature (15/8 degrees C) until maturity, c) raised from cold-primed seeds but grown at optimum temperature (25/18 degrees C) until maturity, d) raised from cold-primed seeds and exposed to low temperature (15/8 degrees C), until maturity. The plants were tested for various traits in the leaves at 0-25 days after exposure to stress. Cold stress resulted in damage to membranes, photosynthetic ability (as loss of chlorophyll, photosystem II function and chlorophyll fluorescence, stomatal conductance), photo-assimilation capacity (as sucrose metabolism), hydration status (as leaf water content), production of osmolytes (as disruption in metabolism of proline, trehalose, glycine betaine, and GABA production) and decreased the redox status of the cells (as decrease in activity and expression of various enzymatic and non-enzymatic antioxidants. Reproductive function was markedly decreased, as was evident from reduction in pollen viability, pollen germination, stigma receptivity and ovule viability). Pod number and seed weight plant(-1) showed 65.5 and 68.4% reduction because of cold stress. The plants raised from cold-primed seeds were remarkable benefitted at reproductive stage, which showed significantly improved pollen and stigmatic function, resulting in considerable recovery of the pod number and seed weight plant(-1). The benefits of the cold priming were possibly related to improved leaf function (hydration status, photosynthetic and carbon fixation ability), which increased the sucrose concentration in the leaves to support the reproductive function, along with enhanced anti-oxidative capacity and osmo-protectants' production, which were significantly more in cold-stressed plants, grown from cold-primed seeds, compared to the controls. Our findings in this regard are novel and suggest an effective strategy to enhance cold tolerance in chickpea.
机译：鹰嘴豆，一种重要的食物豆类，对生殖阶段的冷应激具有很高的敏感性，并且在此阶段的低于20/10℃的温度（昼夜温度）导致生殖部件的发育和功能方面的破坏，导致花卉堕胎，豆荚差和显着的产量损失。在本研究中，鹰嘴豆种子（冷敏感基因型GPF2）在黑暗中在5℃+/- 1摄氏度中冷却30天，然后在15℃下逐渐风干至原始水分含量（ 11-13％）。 11月份在室外环境中的非灌注和灌注种子（温度曲线：11.7摄氏度;平均日/夜间温度; 1300-1500 mm mol m（-2）S（-1）光强度，65-70％的相对湿度），直到发芽和开花的发作（播种后45天）。随后，将植物分离成不同的组，以施加受到受控环境中的治疗方法a）对照（在最佳温度下;在最佳温度（25/18℃）下生长一组植物直至成熟，b）冷应力;在低温（15/8℃）直至成熟度C）的一套植物从冷灌注种子中饲养，但在最佳温度（25/18℃）下生长直至成熟，d）从冷灌注中提升种子并暴露于低温（15/8℃），直至成熟。在暴露于应力后0-25天在叶子中进行各种性状测试植物。冷应力导致膜损伤，光合能力（作为叶绿素，光系统II功能和叶绿素荧光，气孔导率），光同化能力（作为蔗糖代谢），水合状态（作为叶含水含量），渗透剂的生产（由于脯氨酸，海藻糖，甘氨酸甜菜碱和GABA生产中断并降低了细胞的氧化还原状态（作为各种酶促和非酶促抗氧化剂的表达减少。生殖功能显着下降，正如明显的那样降低花粉活力，花粉萌发，颗粒接收和胚珠活力）。 POD编号和种子重量植物（-1）由于冷应激而降低65.5和68.4％。从冷皮种子饲养的植物在生殖阶段受益，显着改善了花粉和耻骨功能显着改善，导致荚数和种子重量植物（-1）的相当大恢复。冷启动的益处可能与改善的叶功能（水合状态，光合和碳固定能力）相关，这增加了叶片中的蔗糖浓度以支持生殖功能，以及增强的抗氧化能力和Osmo保护剂'与对照相比，生产从冷灌注种子生长的冷凝植物中的生产明显更多。我们在这方面的调查结果是新颖的，并提出了一种有效的策略来提高鹰嘴豆的耐寒性。

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