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Exceptional flooding tolerance in the totipotent recalcitrant seeds of Eugenia stipitata

机译:Eugenia Stipitata的Totipotent Rocalctrant种子中的特殊洪水耐受性

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摘要

Eugenia stipitata occurs along rivers in Western Amazonia and produces berry-type fruits with economic potential. Its large recalcitrant (i.e. desiccation-intolerant) seeds have been proposed as a model to study seed stress response, as no apparent differentiation between the embryonic axis and the fused cotyledons are visible. Here, the longevity of submerged seeds was analysed with a view to understanding adaptive mechanisms to seasonal flooding. Submerged seeds began germinating after 2 months. After 1 year, 87 and 96% total germination was reached when seeds were submerged under a water column of 6 cm (where seedlings could emerge from under the water) and 26 cm (where seedlings could not reach the water surface), respectively. Seedling morphology was altered underwater, with short internodes and rudimentary leaf blades, and when submersion was terminated, seedlings transplanted to nursery conditions recovered a normal phenotype. Furthermore, when seedlings were detached from the seeds, the 'resown' seeds produced a second, normal seedling within 9 months. Concentrations of the antioxidant glutathione, which was measured as a stress marker, increased with submersion time in water. Seeds that had developed roots and shoots underwater had higher concentrations of glutathione disulphide than non-germinated seeds, suggesting that the flooding stress was more intense for seedlings than seeds, although more oxidizing cellular redox environments are also consistent with the conditions required for differentiation. Submergence underwater is recommended for storage of the recalcitrant seeds of E. stipitata for up to 1 year.
机译:Eugenia Stipitata沿着西亚马逊西部的河流发生,并产生经济潜力的浆果型水果。已经提出了其大核批量(即干燥 - 不宽容)种子作为研究种子应激反应的模型,因为胚胎轴和熔融子叶之间没有明显的分化。在这里,分析了淹没种子的寿命,以了解对季节性洪水的自适应机制。浸没种子在2个月后开始发芽。 1年后,当在6cm的水柱下浸没种子(其中幼苗从水中出现)时,达到87和96%的总萌发,分别为26厘米(幼苗无法达到水面)。水下幼苗形态改变,短部间和基本叶片,并且当浸没终止时,移植到苗圃条件的幼苗回收了正常的表型。此外,当从种子中脱离幼苗时,“已转移”种子在9个月内产生第二个,正常的幼苗。测量作为应力标记物的抗氧化剂谷胱甘肽的浓度随着水中的浸没时间而增加。在水下开发的根和芽的种子具有比非发芽的种子更高的谷胱甘肽浓度,这表明洪水应力比种子更强烈地剧烈,尽管更多氧化细胞氧化还原环境也与分化所需的条件一致。建议淹没水下水下,用于储存E.Stipitata的核批分种子长达1年。

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