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首页> 外文期刊>Sensor Letters: A Journal Dedicated to all Aspects of Sensors in Science, Engineering, and Medicine >Comparison of Carbon and Platinum Performance as TiO{sub}2 Membrane Substrate Material and Applied to Glucose Biosensor Using Amperometric Readout Circuit
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Comparison of Carbon and Platinum Performance as TiO{sub}2 Membrane Substrate Material and Applied to Glucose Biosensor Using Amperometric Readout Circuit

机译：碳和铂性能作为TiO {Sub} 2膜底物材料的比较，并应用于使用安培读数电路的葡萄糖生物传感器

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摘要

This work proposes an amperometric glucose biosensor based on TiO{sub}2 enzyme-sensitive field effect transistor (EnFET). The sensing film of separated extended-gate field-effect transistor (SEGFET) was produced by screen-printed carbon fabricated on a flexible substrate and E-gun evaporating platinum. Titanium oxide (TiO{sub}2) was used with sol-gel technology or sputtering technology to deposit on the carbon or platinum substrate. Gel entrapment was adopted to fix glucose oxidase on TiO{sub}2 membrane to form enzyme membrane, which was made using enzyme immobilizing technology to establish EnFET. The responding electric currents were measured using a three-electrode cyclic voltammetry (CV) measurement system. Experimental results exhibit that enzyme/TiO{sub}2/carbon and enzyme/TiO{sub}2/Pt show typical CV characteristics adopted three electrodes system. Platinum has large work function, so using it as the TiO{sub}2 conductive substrate owns close zero reaction potentials. Enzyme/TiO{sub}2/carbon shows one reaction peak clearly. Amperometric readout circuit including two operational amplifiers and a resistance was used of enzyme/TiO{sub}2/conductive-layer glucose sensors. The system used enzyme/TiO{sub}2 as working electrode, the counter electrodes (CE) and the reference electrode (RE) which are all TiO{sub}2/conductive-layer. In this experiment, the TiO{sub}2/conductive-layer is conductive, and so can be used as a counter electrode and a reference electrode to deliver the base potential for working electrode. The counter electrodes maintains for keeping a constant potential between RE and CE. The enzyme/TiO{sub}2/Pt results exhibit good linearity for glucose concentrations of between 0 and 400 mg/dL, and the corresponding sensitivity is approximately 74.1 (nV cm{sup}(-2)) (100 mg/dL){sup}(-1) glucose.
机译：该工作提出了基于TiO {Sub} 2酶敏感场效应晶体管（ENFET）的安培葡萄糖生物传感器。通过在柔性基板和电子枪蒸发铂中制造的丝网印刷碳，制备分离的延伸栅场效应晶体管（SEGFET）的传感膜。氧化钛（TiO {Sub} 2）与溶胶 - 凝胶技术或溅射技术一起使用，以沉积在碳或铂基底上。采用凝胶夹带来固定TiO {亚} 2膜上的葡萄糖氧化酶以形成酶膜，其使用酶固定技术进行建立ENFET制备。使用三电极循环伏安法（CV）测量系统测量响应电流。实验结果表明酶/ TiO {亚} 2 /碳和酶/ TiO {Sub} 2 / Pt显示典型的CV特性采用三个电极系统。铂金具有大的功函数，因此使用它作为TiO {Sub} 2导电基板拥有近零的反应电位。酶/ TiO {亚} 2 /碳显示出一种反应峰清楚。包括两个运算放大器和电阻的电流读出电路用于酶/ TiO {ul} 2 /导电层葡萄糖传感器。系统使用酶/ TiO {Sub} 2作为工作电极，反电极（Ce）和作为所有TiO {} 2 /导电层的参考电极（RE）。在该实验中，TiO {Sub} 2 /导电层是导电的，因此可以用作对电极和参比电极以输送工作电极的基极电位。计数器保持用于保持RE和CE之间的恒定电位。酶/ TiO {sub} 2 / pt结果表现出良好的线性度，用于0至400mg / d1之间的葡萄糖浓度，相应的灵敏度约为74.1（NV cm {sup}（ - 2））（100 mg / dl） {sup}（ - 1）葡萄糖。

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