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首页> 外文期刊>Separation and Purification Technology >Membrane processing for purification of R-Phycoerythrin from marine macro-alga, Gelidium pusillum and process integration
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Membrane processing for purification of R-Phycoerythrin from marine macro-alga, Gelidium pusillum and process integration

机译：从海洋宏观藻类，绒毛绒和工艺整合纯化R-植物植物的膜加工

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摘要

Ultrafiltration is widely used for separation and purification of several biomolecules from various sources. However, the performance of ultrafiltration in terms of transmembrane flux, yield and purity vary with biomolecule and process parameters. In the present study, ultrafiltration was employed for the purification of RPhycoerythrin from marine macroalga Gelidium pusillum. Parameters such as molecular weight cut-off (30, 50, 100 and 300 kDa), transmembrane pressure (1, 2 and 4 bar), pH (4, 5, 6 and 7), and ionic strength (control, 0.5 and 1.0 M) were standardised. Ultrafiltration in diafiltration mode and its integration with ammonium sulphate precipitation and aqueous two-phase extraction was also carried out to maximise R-PE purity. In-situ ultrasonication was employed to enhance the transmembrane (permeate) flux. Permeate flux was evaluated employing Hermia's model to have insight and understand membrane fouling that occurs during ultrafiltration. Integration of ammonium sulphate precipitation followed by diafiltration and aqueous two-phase extraction has resulted in 17 fold enrichment in R-PE purity with 61% yield and removal of more than 95% of soluble sugars. In-situ ultrasonication has resulted in increase of transmembrane flux by 10% to 47 L/m(2)/h. Hermia's model based analysis of permeate flux has revealed that cake formation is the predominant type of fouling. Superscript/Subscript Available
机译：超滤广泛用于从各种来源分离和纯化几种生物分子。然而，在跨膜通量，产率和纯度随着生物分子和工艺参数而进行超滤的性能。在本研究中，采用超滤用于从海洋大草原绒毛中纯化抗疱疹。分子量截止（30,50,100和300kDa），跨膜压（1,2和4巴），pH（4,5,6和7）和离子强度（对照，0.5和1.0 m）标准化。还进行了渗滤模式的超滤及其与硫酸铵沉淀和水性两相萃取的整合，以最大化R-PE纯度。采用原位超声，以增强跨膜（渗透物）通量。使用Hermia的模型评估渗透助焊剂具有洞察力和理解在超滤期间发生的膜污染。硫酸铵沉淀随后的渗滤和水性两相萃取的整合导致R-PE纯度富集17倍，产率为61％，除去超过95％的可溶性糖。原位超声会导致跨膜通量增加10％至47L / m（2）/ h。 Hermia对渗透助焊剂的基于模型的分析表明，蛋糕形成是污垢的主要类型。上标/下标可用

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