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Copper complex formed with pyridine rings grafted on cellulose nanofibrous membranes for highly efficient lysozyme adsorption

机译:用吡啶环形成的铜配合物接枝在纤维素纳米纤维膜上,用于高效的溶菌酶吸附

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摘要

The development of separation and purification materials with high adsorption performance and large processing throughput is of great importance. Regenerated cellulose nanofibers membranes (RC-NFM) with average fiber diameter 300 nm were fabricated and functionalized via grafting 4-vinyl pyridine (4-VP) on the cellulose (RC-VP) using a chemically immobilized photo-initiator. The pyridines in RC-VP form complex nanofibrous membranes with Cu(II) to form RC-VP-Cu(II) which enhances selectivity for lysozyme adsorption. The RC-VP-Cu (II) NFM showed superb static adsorption capacity of 800 mg.g(-1) and a high saturated dynamic protein binding capacity of 700 mg.g(-1) toward lysozyme. Besides, benefiting from its stable physical and chemical structures, after 5 cycles of reuses, RC-VP-Cu(II) still showed promising efficiency for lysozyme separation from egg-while samples with lysozyme activity 80% of a synthetic one. The successful synthesis of such intriguing and economic RC-VP-Cu(II) NFM may serve as a promising candidate for the next generation of rapid, massive, and cost-effective separation and purification of proteins.
机译:具有高吸附性能和大加工吞吐量的分离和纯化材料的发展具有重要意义。使用化学固定的光引发剂,通过在纤维素(RC-VP)上通过接枝4-乙烯基吡啶(4-VP)制备具有平均纤维直径300nm的再生纤维素纳米纤维膜(RC-NFM)。 RC-VP中的吡啶形成复合纳米纤维膜的Cu(II)以形成RC-VP-Cu(II),其增强了溶菌酶吸附的选择性。 RC-VP-Cu(II)NFM显示出800mg.g(-1)的一流静态吸附容量,高饱和动态蛋白质结合能力为700mg.g(-1)升。此外,从其稳定的物理和化学结构受益于5次重复循环后,RC-VP-Cu(II)仍然表现出与蛋白的溶菌酶分离的有希望的效率,而溶菌酶活性80%的合成液。这种有趣和经济RC-VP-Cu(II)NFM的成功合成可以作为下一代快速,大规模和经济有效的分离和纯化的蛋白质的有希望的候选者。

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