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首页> 外文期刊>Separation and Purification Technology >Removal of glycerol from enzymatically produced 2-alpha-D-glucosyl-glycerol by discontinuous diafiltration
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Removal of glycerol from enzymatically produced 2-alpha-D-glucosyl-glycerol by discontinuous diafiltration

机译：通过不连续渗滤从酶促制备的2-α-D-葡萄糖基 - 甘油中除去甘油

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摘要

2-alpha-D-Glucosyl-glycerol (2-GG) is a natural glycoside that acts as a biological extremolyte. 2-GG is a promising ingredient for cosmetic products. Industrially, 2-GG is synthesised from sucrose in the presence of a high excess of glycerol using the enzyme sucrose phosphorylase. For commercial applications, the surplus glycerol must be removed and an efficient purification strategy is therefore demanded. Here, we demonstrate nanofiltration for the separation of glycerol from 2-GG. Based on membrane screening performed at varied pressure and temperature, we identified a 150-300 Da cut-off polyamide membrane for the selective removal of glycerol at high permeate throughput. An enzymatically produced 2-GG solution was purified with multicycle discontinuous diafiltration on the litre scale. The process was operated at constant permeate flux (13 kg m(-2)h(-1)) and at a temperature of 30 degrees C. A complete removal of glycerol was achieved after six diafiltration cycles. This was in accordance with the simulation results of a mass balance model. The purity of the product solution increased to 80%, which is comparable with commercially products containing functional carbohydrates. The achieved product recovery was around 85%.
机译：2-α-D-葡萄糖基 - 甘油（2-GG）是一种充当生物氧解的天然糖苷。 2-GG是化妆品的有希望的成分。在工业上，使用酶蔗糖磷酸化酶在高过量过量的甘油存在下从蔗糖中合成2-Gg。对于商业应用，必须去除剩余的甘油，因此需要有效的净化策略。在这里，我们证明了纳米过滤，用于将甘油从2-Gg分离。基于在变化的压力和温度下进行的膜筛选，我们鉴定了一种150-300Da截止聚酰胺膜，用于在高渗透产量下选择性除去甘油。升升纯化了一种酶促产生的2-GG溶液，升高。该方法以恒定的渗透剂（13kg m（-2）H（-1））和30℃的温度运行。在六个渗滤循环后，实现了完全除去甘油。这是根据质量平衡模型的模拟结果。产物溶液的纯度升至80％，与含有官能碳水化合物的商业产品相当。达到的产品回收率约为85％。

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