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首页> 外文期刊>Separation and Purification Technology >Forced vortex assisted liquid phase microextraction for preconcentration and spectrophotometric determination of mefenamic acid in biological samples
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Forced vortex assisted liquid phase microextraction for preconcentration and spectrophotometric determination of mefenamic acid in biological samples

机译：强制涡旋辅助液相微萃取，用于预浓缩和分光光度法的生物样品中梅芬酸的测定

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A new microextraction approach termed forced vortex assisted liquid phase microextraction (FVA-LPME) has been developed and optimized for spectrophotometric determination of mefenamic acid (MFA) as a model compound. FVA-LPME is based on the formation of a rotational vortex of organic phase within aqueous phase and subsequent partition of target analyte between two phases. Rotation of fine layer of organic phase as a solid body within aqueous phase accelerates the mass transfer and improves the extraction efficiency. MFA is extracted into organic phase by FVA-LPME procedure and subsequently derivatized by iodine to form a charge transfer complex for spectrophotometric detection. A response surface methodology (RSM) based on central composite chemometrics design was used for multivariate optimization of the effects of three different parameters influencing the extraction efficiency. Under the optimal conditions, the calibration curve was linear in the range of 10-900 ng mL(-1) of MFA with a R-2 of 0.998. The repeatability and reproducibility (RSD%) for 200 ng mL(-1) MFA were 2.6% and 3.3%, respectively and limit of detection limit (S/N = 3) was estimated to be 3.1 ng mL(-1). The results demonstrated that the developed method is an accurate, inexpensive, rapid and reliable sample pretreatment method that gives very good enrichment factor and detection limit for extracting and determining MFA in biological samples. (C) 2016 Elsevier B.V. All rights reserved.
机译：已经开发出一种新的微萃取方法，其被强制涡旋辅助液相微萃取（FVA-LPME），并针对分光光度法测定Mefenamic酸（MFA）作为模型化合物的分光光度法。 FVA-LPME基于在水相中形成有机相的旋转涡流和随后的两相之间的靶分析物分配。有机相的细层的旋转作为水相中的固体加速了传质并提高了提取效率。通过FVA-LPME程序将MFA萃取到有机相中，随后通过碘衍生化以形成用于分光光度检测的电荷转移复合物。基于中央复合化学计量器设计的响应面方法（RSM）用于多变量优化影响提取效率的三种不同参数的效果。在最佳条件下，校准曲线在10-900 ng ml（-1）的MFA的范围内，R-2为0.998。 200ng ml（-1）MFA的可重复性和再现性（RSD％）为2.6％和3.3％，分别估计检测极限（S / N = 3）的限制为3.1ng ml（-1）。结果表明，开发方法是一种准确，廉价，快速可靠和可靠可靠的样品预处理方法，其为在生物样品中提取和确定MFA的提取和确定MFA提供了非常好的富集因子和检测限。 （c）2016年Elsevier B.v.保留所有权利。

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