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首页> 外文期刊>Slovenian Veterinary Research >OCCURRENCE, MOLECULAR IDENTIFICATION AND ANTIBIOTIC RESISTANCE PROFILING OF MYCOPLASMA GALLISEPTICUM AND MYCOPLASMA SYNOVIAE FROM CHRONIC RESPIRATORY DISEASE CASES IN POULTRY BIRDS AND FARM ENVIRONMENT
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OCCURRENCE, MOLECULAR IDENTIFICATION AND ANTIBIOTIC RESISTANCE PROFILING OF MYCOPLASMA GALLISEPTICUM AND MYCOPLASMA SYNOVIAE FROM CHRONIC RESPIRATORY DISEASE CASES IN POULTRY BIRDS AND FARM ENVIRONMENT

机译：从家禽鸟类和农场环境中慢性呼吸道疾病慢性呼吸道疾病的慢性呼吸道疾病的发生，分子鉴定和抗生素抗性分析

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Avian mycoplasmosis is an important risk for commercial poultry production leading to enormous losses in terms of disease and productivity. The main causative agents are Mycoplasma gallisepticum and Mycoplasma synoviae. To study the variable degree of resistance to commonly prescribed and used antibiotics in mycoplasmosis, a total of 115 samples including tissue specimen and swabs were collected from chronic respiratory disease (CRD) cases of broiler and layer birds and their contaminated farm environment. The samples were directly passaged into the Brain Heart Infusion broth (supplemented with 10 % horse serum, NAD, cysteine, penicillin and thallium acetate). Positive samples were transferred to Brain Heart Infusion agar (Difco) for the isolation of Mycoplasma spp. while negative samples were declared after the third passage. Of the samples, 61.5% were found positive for Mycoplasma spp., which were recovered mostly after second passage. Out of total culture positive cases, Mycoplasma gallisepticum (MG) was identified in 62% cases and Mycoplasma synoviae (MS) in 38%, as confirmed through Polymerase Chain Reaction (PCR) using specific primers. The MG and MS isolates showed variable degrees of sensitivity against the commercially available drug of choice, tylosin. The highest Minimum Inhibitory Concentration (MIC) of enrofloxacin (112.38 +/- 4.34 mu g/ml) was recorded against MG, followed by tetracyclin (91.58 +/- 4.66 mu l/ml), gentamicin (54.33 +/- 2.98 mu g/ml), spiromicin (52.23 +/- 3.99 mu g/ml) and tylosin (52.58 +/- 2.69 mu g/ml). The highest MIC for enrofloxacin (168.24 +/- 3.82 mu g/ml) was recorded against MS followed by tetracyclin (115.48 +/- 2.62 mu g/ml), spiromicin (95.96 +/- 2.17 mu g/ml), tylosin (84.84 +/- 2.56 mu g/ml) and gentamicin (46.4 +/- 2.18 mu g/ml). Multiplex PCR is a time tested tool for the molecular diagnosisand confirmation of Mycoplasma species.
机译：Avian Mycoplasmosis是商业家禽产量的重要风险，导致疾病和生产力方面的巨大损失。主要致病剂是支原体脑腹和支原体嗜睡剂。为了研究对mycoplasmosis的常见规定和使用抗生素的可变程度，共有115个样品，包括肉鸡和层鸟类的慢性呼吸道疾病（CRD）病例及其受污染的农场环境。将样品直接传递到脑心脏输注肉汤中（补充10％的马血清，NAD，半胱氨酸，青霉素和醋酸铊）。将阳性样品转移到脑心脏输注琼脂（DIFCO）中以分离支原体SPP。虽然在第三段段落之后宣布阴性样品。对于试杆菌SPP，发现了61.5％的阳性。第二段通过后主要回收。除了总培养阳性病例中，通过使用特异性引物通过聚合酶链式反应（PCR）确认，在62％病例和支原体中的嗜型嗜碱（Mg）中鉴定了支原体Gallisepticum（Mg）。 Mg和MS分离物显示出可变的敏感性，对市售的选择药物，Tylosin。对Mg记录富含氧氟沙星（112.38 +/-4.34μg/ ml）的最高最小抑制浓度（MIC），其次是四环素（91.58 +/-4.66μl/ ml），庆大霉素（54.33 +/- 2.98 mu g / ml），螺旋蛋白（52.23 +/- 3.99 mu g / ml）和tylosin（52.58 +/- 2.69 mu g / ml）。富含氧氟沙星的最高麦克风（168.24 +/-3.82μmg/ ml）对MS进行记录，然后用四环素（115.48 +/-2.62μmg/ ml），螺旋蛋白（95.96 +/- 2.17 mu g / ml），tylosin（ 84.84 +/- 2.56 mu g / ml）和庆大霉素（46.4 +/- 2.18 mu g / ml）。多重PCR是用于分子诊断的时间测试工具，用于支原体的分子诊断。

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