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首页> 外文期刊>South African Journal of Botany >Phytochemical screening, in vitro evaluation of the antimicrobial, antioxidant and cytotoxicity potentials of Grewia lasiocarpa E. Mey. ex Harv.
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Phytochemical screening, in vitro evaluation of the antimicrobial, antioxidant and cytotoxicity potentials of Grewia lasiocarpa E. Mey. ex Harv.

机译：植物化学筛查，对抗菌剂，抗氧化剂和细胞毒性潜力的体外评价，梅伊。 ex harv。

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The plant Grewia lasiocarpa E. Mey. ex Harv. (FamilyMalvaceae sensu lato), is relatively understudied. However, plants of this genus are well known for their phyto-medicinal and ethnopharmacological values. This study evaluated the preliminary phytochemistry, Fourier transform infrared (FTIR), Gas chromatography-mass spectroscopy analysis, total phenolic contents, in vitro antibacterial and antioxidant activities of the crude hexane, chloroform andmethanol extracts of the leaves and stembark of G. lasiocarpa. The antimicrobial activity was investigated by agar-well diffusion method against selected clinical strains. The in vitro antioxidant assay of the crude extracts was done using 2, 2'-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and ferric reducing antioxidant power (FRAP). The phenolic content was measured using Folin-Ciocalteu's assay. The cytotoxicity of the crude extracts was tested using MTT assay on HEK293, HeLa and MCF-7 cell lines. In all the extracts, tannins, flavonoids, phenols and carbohydrates are among the primary and secondary metabolites confirmed to be present, while the FT-IR spectroscopic analysis of all extracts confirmed the presence of alkanes, aromatic and alkyl halides in all extracts of the leaves and stembark. Among, the major compounds found in all extracts phytol, friedelin, n-hexadecanoic acid were present in the leaves, while in the stem bark, hexadecane, lup-20(29)-en-3-ol, acetate and hexanedioic acidwere present. The methanol extracts of the leaves and stembark had the lowest IC50 value of 0.02 mu g/mL and 1.34 exp.(-5) mu g/mL (DPPH), while for FRAP the methanol extract of the leaves and chloroform extract of the stem had the lowest IC50 value of 9.76 and 12 mu g/mL, respectively. The methanol extract of the leaves and stembark had the highest phenolic content of 132.82 +/- 0.22 and 171.12 +/- 0.39 mgGAE/g dried extract. Most of the isolates showed varying degree of sensitivity to the extracts, with the hexane extracts of the leaves and stem bark having a remarkable inhibitory effect against Methicillin-resistant Staphylococcus aureus (ACCC 14026). The extracts were relatively not cytotoxic to HEK293. The results are suggestive that G. lasiocarpa leaves and stem bark are rich in bioactive compounds which could be a potential source of natural antioxidants and antibacterial agent for the treatment of diseases relating to the pathogenic bacteria studied. (C) 2019 SAAB. Published by Elsevier B.V. All rights reserved.
机译：植物Grewia Lasiocarpa E. Mey。 ex harv。 （FamilyMalvaceae Sensu Lato）相对描述。然而，这种属的植物众所周知，其植物药物和民族医药值是众所周知的。本研究评估了初步的植物化学，傅里叶变换红外（FTIR），气相色谱 - 质谱分析，总酚类含量，体外抗菌和抗氧化剂和抗氧化剂和抗氧化活性的粗己烷，氯仿和甲甲醇萃取物的叶片和Lasiocarpa的茎。通过对选定的临床菌株进行琼脂孔扩散法研究了抗微生物活性。使用2,2'-二苯基-1-富铬酰基（DPPH）清除活性和耐氧化碳抗氧化能力（FRAP）进行粗提取物的体外抗氧化试验。使用Folin-Ciocalteu测定法测量酚含量。使用HEK293，HELA和MCF-7细胞系上使用MTT测定测试粗提取物的细胞毒性。在所有提取物中，单宁，黄酮类化合物，酚和碳水化合物是迄今为止存在的初级和次生代谢物之一，而所有提取物的FT-IR光谱分析证实了叶片的所有提取物中的烷烃，芳族和烷基卤化物和Stembark。在所有提取物植物中发现的主要化合物，Friedelin，Friedelin，N-十六烷烯酸中存在，而存在于茎树皮，十六烷，LUP-20（29）-En-3-醇，乙酸盐和己二核酸中存在。叶片和茎干的甲醇提取物具有0.02μg/ ml的最低IC50值和1.34 exp。（ - 5）mu g / ml（dpph），而用于叶子的甲醇提取物和茎的氯仿提取物具有9.76和12μg/ ml的最低IC50值。叶片和茎干的甲醇提取物的酚含量最高为132.82 +/- 0.22和171.12 +/- 0.39 mgGae / g干燥提取物。大多数分离物向提取物显示出不同程度的敏感性，叶片和茎鲨的己烷提取物具有显着的抑制抑制抑制甲氧西林金黄色葡萄球菌（ACCC 14026）。提取物对HEK293相对没有细胞毒性。结果表明，G. Lasiocarpa叶和茎树皮富含生物活性化合物，其可以是天然抗氧化剂和抗菌剂的潜在来源，用于治疗所研究的病原细菌的疾病。 （c）2019 Saab。 elsevier b.v出版。保留所有权利。

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