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首页> 外文期刊>South African Journal of Plant and Soil >Multiplex-ready PCR assay of SSR marker diversity among quality protein maize inbred parental lines
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Multiplex-ready PCR assay of SSR marker diversity among quality protein maize inbred parental lines

机译：质量蛋白质玉米血液血液中SSR标记多样性SSR标记多样性的多路复用PCR检测

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摘要

Phenotypic markers have long been used for classification of maize (Zea mays) cultivars. However, because of shortcomings in detecting differences among closely related genotypes, coupled with sensitivity to environmental conditions, molecular markertechniques have been employed in the determination of relationships among parental genotypes at the DNA level. In this study, simple sequence repeat (SSR) multiplex-ready PCR was used to determine the diversity among 24 quality protein maize (QPM) inbredlines. Analysis of SSR loci of the 24 QPM lines yielded 101 scorable alleles with an average of 5.05 alleles per locus. The highest polymorphic information content (0.77) was observed in di-repeat motifs (Bng 11714) and the least (0.15) in tetra-nucleotide repeat motifs (Phi 053). The expected and observed mean heterozygosities were 0.62 and 0.11, respectively. These values showed deviation from the Hardy–Weinberg equilibrium, an affirmation of genetic diversity among the maize accessions. UPGMA cluster analysis resolved four major clusters with grouping patterns consistent with ancestry information. The results indicate that the multiplex-ready PCR technique could avail breeders in developing countries, especially Africa where research funding is limited, the opportunity of making use of marker techniques in breeding programmes because it is highly economical and time efficient.
机译：表型标志物只用于玉米（Zea Mays）品种的分类。然而，由于检测密切相关基因型之间的差异的缺点，与对环境条件的敏感性相结合，所以在DNA水平的父母基因型中的关系中使用了分子标记。在该研究中，使用简单的序列重复（SSR）多重就绪PCR用于确定24质量蛋白质玉米（QPM）中的多样性。分析24 QPM系列的SSR基因座产生101个可批量的等位基因，平均每位轨迹为5.05等位基因。在二重复基序（BNG 11714）中观察到最高多态性信息含量（0.77），并且Tetra核苷酸重复基序（PHI 053）中的最低（0.15）。预期和观察到的平均杂合子分别为0.62和0.11。这些值显示出偏离Hardy-Weinberg均衡，这是玉米中遗传多样性的肯定。 UPGMA集群分析解决了四个主要群集，其中包含与祖先信息一致的分组模式。结果表明，多路复用的PCR技术可以利用发展中国家的育种者，特别是非洲研究资金有限，利用育种计划中使用标记技术的机会，因为它具有高度经济和时间效率。

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