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DNA Yield and Turtle Handling Time: Buccal Swabs Versus Blood Samples from Red-eared Sliders and Eastern Musk Turtles

机译:DNA产量和乌龟处理时间:口腔拭子与红耳滑块和东麝龟的血液样本

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摘要

Conventional DNA sampling techniques for reptiles and amphibians are often invasive and difficult to perform in the field. Other, less invasive methods often pose issues with DNA contamination and low DNA yields. Few, if any, studies explicitly quantify handling time, despite being an important source of stress to the study species. Here, we compare handling time and DNA yield for 3 sampling methods (choanal, a general buccal sample excluding the choana, and blood) for Trachemys scripta elegans (Red-eared Slider) and Sternotherus odoratus (Eastern Musk Turtle). Buccal and blood samples had significantly higher DNA yields than choanal samples, but blood draws took substantially less time and personnel to collect than either mouth-sampling technique. Where possible, we recommend blood collection for DNA analyses in turtles.
机译:爬行动物和两栖动物的常规DNA采样技术通常是侵入性的,并且难以在该领域进行。 其他,较少的侵入性方法经常造成DNA污染和低DNA产量的问题。 少数,如果有的话,研究明确地量化了处理时间,尽管是对研究物种的重要压力来源。 在此,我们比较3种取样方法的处理时间和DNA产量(Chooanal,除了Choana的一般口腔样本以及血液中的一般口腔样本,以及血液),用于Trachemys Scripta elegans(红耳滑块)和sternothothothothotorus(东麝龟)。 口腔和血液样本显着较高的DNA产量比Choanal样品显着较高,但血迹比口腔采样技术收集的时间和人员显着更少。 在可能的情况下,我们建议在海龟中进行DNA分析的血液收集。

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