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首页> 外文期刊>Systematic and Applied Microbiology >Mesorhizobium intechi sp. nov. isolated from nodules of Lotus tenuis in soils of the Flooding Pampa, Argentina
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Mesorhizobium intechi sp. nov. isolated from nodules of Lotus tenuis in soils of the Flooding Pampa, Argentina

机译：Mesorhizobium Intechi Sp。 11月。 从莲花植物的结节隔绝在洪水普查，阿根廷土壤的土壤

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摘要

Three symbiotic nitrogen-fixing bacteria (BD68(T), BD66 and BD73) isolated from root nodules of Lotus tenuis in lowland soils of the Flooding Pampa (Argentina), previously classified as members of the Mesorhizobium genus, were characterized in this study. Phylogenetic analysis of their 16S rRNA gene sequences showed a close relationship to M. japonicum MAFF 303099(T), M. erdmanii USDA 3471(T), M. carmichaelinearum ICMP 18942(T), M. opportunistum WSM 2975(T) and M. jarvisii ATCC 33699(T), with sequence identities of 99.72%-100%. Multilocus sequence analysis of other housekeeping genes revealed that the three isolates belonged to a phylogenetically distinct clade within the genus Mesorhizobium. Strain BD68(T) was designated as the group representative and its genome was fully sequenced. The average nucleotide identity and in silico DNA-DNA hybridization comparisons between BD68(T) and the most related type strains showed values below the accepted threshold for species discrimination. Phenotypic and chemotaxonomic features were also studied.
机译：在本研究中，从洪水普及（阿根廷）的低地土壤中，从莲花毒素（阿根廷）的低地土壤中分离出的三种共生氮固定细菌（BD68（T），BD66和BD73），其特征在本研究中。其16S rRNA基因序列的系统发育分析显示出与M. japonicum maff 303099（t），m。erdmanii usda 3471（t），m. carmichaelinearum Icmp 18942（t），m。机会组织wsm 2975（t）和m 。Jarvisii ATCC 33699（T），序列标识为99.72％-100％。其他内政基因的多点序列分析显示，三个分离株属于叶氏菊属植物属中的系统源性不同的疏水板。将BD68（T）指定为组代表性，其基因组完全测序。 BD68（T）和最相关的型菌株之间的平均核苷酸同一性和硅DNA-DNA杂交比较显示出物种鉴别的可接受阈值以下的值。还研究了表型和趋化性的特征。

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