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Vagococcus teuberi sp nov., isolated from the Malian artisanal sour milk fene

机译:vagocccus teuberi sp 11月。,从马里羚羊酸奶fene隔离

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Ten bacterial isolates belonging to the genus Vagococcus were obtained from Malian sour milk fene produced from spontaneously fermented cow milk. However, these isolates could not be assigned to a species upon initial comparative 16S rRNA gene sequence analysis and were therefore further characterized. Rep-PCR fingerprinting of the isolates yielded four strain clusters represented by strains CG-21(T) (=DSM 21459(T)), 24CA, CM21 and 9H. Sequence identity of the 16S rRNA gene of DSM 21459(T) to its closest relative species Vagococcus penaei was 97.9%. Among the four rep strain clusters, DSM 21459(T) and 24CA shared highest 16S rRNA gene sequence identity of 99.6% while CM21 and 9H shared 98.6-98.8% with DSM 21459(T) and V. penaei CD276(T). DSM 21459(T) and 24CA were thus subjected to a polyphasic typing approach. The genome of DSM 21459(T) featured a G + C content of 34.1 mol% for a 2.17-bp chromosome and a 15-kbp plasmid. Average nucleotide identity (ANI) of DSM 21459(T) to Vagococcus fluvialis bH819, V. penaei CD276(T) were 72.88%, 72.63%, respectively. DNA-DNA hybridization (DDH) similarities of strain DSM 21459(T) to other Vagococcus species were &42.0%. ANI and DDH findings strongly supported the 16S rRNA gene phylogenetic tree delineations. The fatty acid patterns of DSM 21459(T) was palmitic acid (C (16:0), 24.5%), oleic acid (C (18:1-omega 9c), 32.8%), stearic acid (C (18:0), 18.9%). General physiological characterization of DSM 21459(T) and 24CA were consistent with those of the genus Vagococcus. Strain DSM 21459(T) and further strains are therefore considered to belong to a novel species, for which the nomenclature Vagococcus teuberi sp. nov. is proposed. The type strain is named CG-21(T) (=DSM 21459(T) and LMG 24695(T)). (C) 2017 Elsevier GmbH. All rights reserved.
机译:从自发发酵的牛奶生产的马里酸奶乳制物中获得了属于恶魔属的10个细菌分离物。然而,在初始比较16S rRNA基因序列分析中,不能将这些分离物分配给物种,因此进一步表征。分离物的Rep-PCR指纹纹理由菌株CG-21(T)(= DSM 21459(T)),24CA,CM21和9H表示的四种应变簇。 DSM 21459(T)的16S rRNA基因的序列同一性至其最近的相对物种Vagococcus Penaei为97.9%。在四个rep应变簇中,DSM 21459(T)和24CA共享最高的16S RRNA基因序列同一性为99.6%,而CM21和9H与DSM 21459(T)和V.Penaei CD276(T)共用98.6-98.8%。因此,对DSM 21459(T)和24CA进行多相打字方法。 DSM 21459(T)的基因组特征在2.17-BP染色体和15-kbp质粒的G + C含量为34.1mol%。 DSM 21459(T)的平均核苷酸同一性(ANI)对vagococcus flyvialis BH819,V.Penaei CD276(T)分别为72.88%,72.63%。 DNA-DNA杂交(DDH)菌株DSM 21459(T)与其他恶作剧物种的相似性& 42.0%。 ANI和DDH调查结果强烈支持16S rRNA基因系统发育树划清症。 DSM 21459(T)的脂肪酸图案是棕榈酸(C(16:0),24.5%),油酸(C(18:1-ω9C),32.8%)硬脂酸(C(18:0) ),18.9%)。 DSM 21459(T)和24CA的一般生理表征与Vagocccus属的那些符合。因此,菌株DSM 21459(T)和其他菌株被认为属于一种新颖的物种,其中名称迷迭香孢子SP。 11月。提出。类型应变名为CG-21(T)(= DSM 21459(T)和LMG 24695(T))。 (c)2017 Elsevier GmbH。版权所有。

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