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首页> 外文期刊>Parasitology >The latest FAD – Faecal antibody detection in cattle. Protocol and results from three UK beef farms naturally infected with gastrointestinal nematodes
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The latest FAD – Faecal antibody detection in cattle. Protocol and results from three UK beef farms naturally infected with gastrointestinal nematodes

机译：最新的粪便粪便抗体检测。 三个英国牛肉农场的议定书和结果自然感染胃肠道线虫

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摘要

Antibodies at gastrointestinal mucosal membranes play a vital role in immunological protection against a range of pathogens, including helminths. Gastrointestinal health is central to efficient livestock production, and such infections cause significant losses. Fecal samples were taken from 114 cattle, across three beef farms, with matched blood samples taken from 22 of those animals. To achieve fecal antibody detection, a novel fecal supernatant was extracted. Fecal supernatant and serum samples were then analysed, using adapted enzyme-linked immunosorbent assay protocols, for levels of total immunoglobulin (Ig)A, IgG, IgM, and Teladorsagia circumcincta-specific IgA, IgG, IgM and IgE (in the absence of reagents for cattle-specific nematode species). Fecal nematode egg counts were conducted on all fecal samples. Assays performed successfully and showed that IgA was the predominant antibody in fecal samples, whereas IgG was predominant in serum. Total IgA in feces and serum correlated within individuals (0.581, P = 0.005), but other Ig types did not. Results support the hypothesis that the tested protocols are an effective method for the non-invasive assessment of cattle immunology. The method could be used as part of animal health assessments, although further work is required to interpret the relationship between results and levels of infection and immunity.
机译：胃肠粘膜膜的抗体在免疫学中起着至关重要的作用，免疫保护免受一系列病原体，包括蠕虫。胃肠道健康是有效的牲畜生产中的核心，这种感染会导致显着的损失。粪便样本从114个牛，穿过三个牛肉农场，其中匹配的血液样本从这些动物中的22种。为了达到粪便抗体检测，提取了一种新型粪便上清液。然后使用适应的酶联免疫吸附测定方案分析粪便上清液和血清样品，用于总免疫球蛋白（Ig）A，IgG，IgM和Teladoragia的Circualcationa特异性IgA，IgG，IgM和IgE（在没有试剂的情况下对于牛特异性线虫物种）。在所有粪便样品上进行粪便线虫蛋计数。成功进行的测定并表明IgA是粪便样品中的主要抗体，而IgG在血清中占主导地位。粪便中的总IgA和血清中的个体内相关（0.581，p = 0.005），但其他IG类型没有。结果支持测试方案是对牛免疫学无侵入评估的有效方法的假设。该方法可作为动物健康评估的一部分，尽管需要进一步的工作来解释结果和感染水平与抗扰度之间的关系。

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