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首页> 外文期刊>Parasitology >Molecular epidemiology of Cryptosporidium spp. in dairy cattle in Guangdong Province, South China
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Molecular epidemiology of Cryptosporidium spp. in dairy cattle in Guangdong Province, South China

机译：Cryptioporidium SPP的分子流行病学。 在南方广东省的奶牛牛

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摘要

To determine the prevalence of Cryptosporidium in dairy cattle in Guangdong Province, South China, 1440 fecal samples were collected from 10 farms and screened for Cryptosporidium with PCR. The overall prevalence of Cryptosporidium was 4.38% (63/1440), and the infection rates in preweaned calves, postweaned calves, heifers and adults were 6.4% (19/297), 6.19% (33/533), 1.48% (4/271) and 2.06% (7/339), respectively. Three Cryptosporidium species, Cryptosporidium andersoni (n = 33), Cryptosporidium bovis (n = 22) and Cryptosporidium ryanae (n = 8) were detected by DNA sequence analysis of the 63 positive samples, and C. andersoni was identified as the most common species on the dairy cattle farms. In preweaned calves, C. bovis was the most prevalent species (9/19, 47.4%). In contrast, C. andersoni was the predominant species (19/33, 57.6%) in postweaned calves and the only species found in heifers and adults. The zoonotic species Cryptosporidium parvum was not detected in this study. Twenty-four C. andersoni isolates were successfully classified into three multilocus sequence typing (MLST) subtypes. MLST subtype A4,A4,A4,A1 was the predominant subtype, and MLST subtype A2,A5,A2,A1, previously found in sheep, was detected in cattle for the first time. A linkage disequilibrium analysis showed that the C. andersoni isolates had a clonal genetic population structure. However, further molecular studies are required to better understand the epidemiology of Cryptosporidium in Guangdong.
机译：为了确定广东省奶牛牛的密码孢子率普及，从10个农场收集了1440个粪便样品，并筛选了PCR的密码孢子虫。密码孢子虫的总体流行率为4.38％（63/1440），预期的小牛，后牛犊，小牛母牛和成人的感染率为6.4％（19/297），6.19％（33/533），1.48％（4 / 271）分别为2.06％（7/339）。通过63个阳性样品的DNA序列分析检测到三个隐孢子虫（n = 33），密码孢子虫（n = 33），密码孢子虫（n = 22）和黑麦孢菌（n = 8），并鉴定为最常见的种类在奶牛场。在预期的小牛中，C.Bovis是最普遍的物种（9/19,47.4％）。相比之下，C. Andersoni是断奶牛犊中的主要物种（19/33,57.6％），唯一的小母牛和成年人中唯一的物种。在本研究中未检测到动物孢子虫孢子座上植物。二十四个C.和氏种分离物被成功分为三个多层序列键入（MLST）亚型。 MLST亚型A4，A4，A4，A1是主要的亚型，并且在羊中发现以前在绵羊中发现的MLST亚型A2，A5，A2，A1。连锁不平衡分析表明，C. Andersoni分离物具有克隆遗传群结构。然而，需要进一步的分子研究来更好地了解广东密码孢子虫的流行病学。

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