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首页> 外文期刊>Parasitology >Development and characterization of monoclonal antibodies against Besnoitia besnoiti tachyzoites
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Development and characterization of monoclonal antibodies against Besnoitia besnoiti tachyzoites

机译：对BesnoitiitiTaite的单克隆抗体的发展与表征

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This is the first report on the development and characterization of eight monoclonal antibodies (MABs) generated against whole- and membrane-enriched tachyzoite extracts of the apicomplexan parasite Besnoitia besnoiti. Confocal laser scanning immunofluorescence microscopy was used to localize respective epitopes in B. besnoiti tachyzoites along the lytic cycle. A pattern compatible with dense granule staining was observed with MABs 2.A.12, 2.F.3 and 2.G.4, which could be confirmed by immunogold electron microscopy for MABs 2.A.12 and 2.F.3. In particular, MABs 2.F.3 and 2.G.4 were secreted during early invasion, proliferation and egress phases. MABs 3.10.8 and 5.5.11 labelled the tachyzoite surface, whilst MABs 1.17.8, 8.9.2 and 2.G.A recognized the apical tip, which is reminiscent for microneme localization. Besides, the epitopes recognized by the latter two (MABs 8.9.2 and 2.G.A) exhibited a redistribution from the anterior part across the parasite surface towards the posterior end during invasion. Most MABs developed were genus-specific. Indeed, the MABs cross-reacted neither with T. gondii nor with N. caninum tachyzoites. In summary, we have generated MABs that will be useful to study the key processes in the lytic cycle of the parasite and with additional promising diagnostic value. However, the molecular identity of the antigens recognized remains to be elucidated.
机译：这是第一份关于ApicoMemplean寄生虫Besnoitii的全部和膜的富含Tachyzoite提取物产生的八种单克隆抗体（mAb）的开发和表征的报告。共聚焦激光扫描免疫荧光显微镜用于沿着裂变循环局部化B.BESNOITI Tachyzoites的各自表位。用MAb 2.A.12,2.F.3和2.G.4观察到与致密颗粒染色相容的图案，其可以通过免疫元源电子显微镜用于MAbs 2.a.12和2.f.3 。特别是，在早期侵袭，增殖和出口阶段期间分泌mAb 2.F.3和2.G.4。 MABS 3.10.8和5.5.11标有Tachyzoite表面，而MAbs 1.17.8,8.9.2和2.g.a认识到顶端尖端，这使得可以让人联络。此外，后两种（MAb 8.9.2和2.G.a）认识到的表位从寄生过程中朝向后端的前部展示了从前部的前部重新分布。大多数MAB开发的是特异性属。实际上，mAbs既不与吉隆艇都没有反应，也没有与N. caninum tachyzoites。总之，我们产生了对研究寄生虫裂变循环中的关键过程和额外有前途的诊断价值有用的MAB。然而，识别的抗原的分子标识仍有待阐明。

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