...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Parasitology >Molecular evidence of prevalent dual piroplasma infections in North American raccoons (Procyon lotor)
【24h】

Molecular evidence of prevalent dual piroplasma infections in North American raccoons (Procyon lotor)

机译：北美浣熊（Procyon Lotor）中普遍的双重子血药物感染的分子证据

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Based on 18S rRNA sequence analyses 2 distinct genotypes of piroplasms have been described in raccoons. One genotype resides in the Babesia sensu stricto clade and the other in the Babesia microti-Xike. clade. Since these organisms appear morphologically indistinguishable, it is unclear which strain is responsible for the majority of the infections in raccoons. In order to overcome these limitations we performed a molecular survey of raccoons using polymerase chain reaction assays specific for each genotype. We tested blood samples from 41 wild raccoons trapped in eastern North Carolina using PCR assays and found that 95 % (39/41) had detectable piroplasm DNA. Ninety percent (37/41) of the samples contained Babesia sensu stricto DNA and 83 % (34/41) samples contained Babesia microti-like DNA. DNA from both genotypes was present in 76% (31/41) samples suggesting a very high rate of co-infections. The presence of dual piroplasma infections in carnivores appears to be an uncommon finding. This study highlights the need for molecular assays for the accurate identification of piroplasma. Further studies are indicated to investigate the ability of these parasites to infect domestic animals as well as their zoonotic potential.
机译：基于18S的RRNA序列分析，在RACOONS中描述了两种分析的单丙烯酶。一种基因型在Babesia Sensu Stictimo Clade和Babesia Microti-xike中的另一个基因型。思工。由于这些生物在形态上难以区分，因此目前尚不清楚哪种菌株对浣熊的大部分感染负责。为了克服这些限制，我们使用针对每个基因型的特异性的聚合酶链反应测定进行raccoons的分子调查。我们使用PCR测定从41次捕获的41个野生浣熊中测试了血液样本，发现95％（39/41）具有可检测的粒子粒子DNA。含有Babesia Sensu的样品的百分之九十（37/41）和83％（34/41）样品含有Babesia microoti样DNA。来自两个基因型的DNA在76％（31/41）样本中存在，表明具有非常高的共感染率。在食肉动物中存在双重子血药物感染的存在似乎是一种罕见的发现。本研究突出了用于精确鉴定的分子测定的需求。表明进一步的研究探讨了这些寄生虫感染了家畜以及它们的动物潜力的能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号