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首页> 外文期刊>Parasitology >A retrospective study comparing agar plate culture, indirect immunofluorescence and real-time PCR for the diagnosis of Strongyloides stercoralis infection
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A retrospective study comparing agar plate culture, indirect immunofluorescence and real-time PCR for the diagnosis of Strongyloides stercoralis infection

机译：一种回顾性研究，比较琼脂平板培养，间接免疫荧光与实时PCR诊断φ睾丸的诊断STERCORALIS感染

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Strongyloides stercoralis is a parasite that can cause death in immunocompromised people. A proper diagnosis is hence essential. The real-time polymerase-chain reaction (RT-PCR) is a novel, promising diagnostic method, that detects the DNA of the parasite in stool samples. In this retrospective study, we compared the sensitivity of agar plate coproculture (APC), an in-house immunofluorescence test (IFAT) and an in-house RT-PCR for the diagnosis of S. stercoralis infection. The study sample was composed by 223 samples. Samples resulting positive to APC, IFAT and RT-PCR were 20, 140 and 25, respectively. When sensitivity was calculated against a composite reference standard, serology confirmed the best performance (sensitivity 95%), followed by RT-PCR (57%) and APC (45%). In conclusion, in a non-endemic setting, serology is the best screening method, while the combination of APC and RT-PCR does not seem a reasonable approach to increase sensitivity. Both methods can have a role as confirmatory tests for selected cases.
机译：Strickyloides Stercoralis是一种寄生虫，可导致免疫因素的人死亡。因此，适当的诊断是必要的。实时聚合酶链反应（RT-PCR）是一种新颖的，有希望的诊断方法，可检测寄生虫的DNA在粪便样品中。在这项回顾性研究中，我们将琼脂平殖民培养（APC）的敏感性与内部免疫荧光试验（IFAT）和内部RT-PCR进行了比较，用于诊断S. Stercoralis感染的内部RT-PCR。研究样品由223个样品组成。产生阳性至APC，IFAT和RT-PCR的样品分别为20,140和25。当根据复合参考标准计算敏感性时，血清学证实了最佳性能（灵敏度95％），然后是RT-PCR（57％）和APC（45％）。总之，在非特有的环境中，血清学是最好的筛查方法，而APC和RT-PCR的组合似乎并不是一种合理的方法来提高敏感性。两种方法都可以具有作为所选病例的确认测试的作用。

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