...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Parasitology >Detecting hybridization in African schistosome species: does egg morphology complement molecular species identification?
【24h】

Detecting hybridization in African schistosome species: does egg morphology complement molecular species identification?

机译：检测非洲血吸虫物种中的杂交：卵形态是否补体分子种鉴定？

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Hybrid parasites may have an increased transmission potential and higher virulence compared to their parental species. Consequently, hybrid detection is critical for disease control. Previous crossing experiments showed that hybrid schistosome eggs have distinct morphotypes. We therefore compared the performance of egg morphology with molecular markers with regard to detecting hybridization in schistosomes. We studied the morphology of 303 terminal-spined eggs, originating from 19 individuals inhabiting a hybrid zone with natural crosses between the human parasite Schistosoma haematobium and the livestock parasite Schistosoma bovis in Senegal. The egg sizes showed a high variability and ranged between 92.4 and 176.4 mu m in length and between 35.7 and 93.0 mu m in width. No distinct morphotypes were found and all eggs resembled, to varying extent, the typical S. haematobium egg type. However, molecular analyses on the same eggs clearly showed the presence of two distinct partial mitochondrial cox1 profiles, namely S. bovis and S. haematobium, and only a single nuclear ITS rDNA profile (S. haematobium). Therefore, in these particular crosses, egg morphology appears not a good indicator of hybrid ancestry. We conclude by discussing strengths and limitations of molecular methods to detect hybrids in the context of high-throughput screening of field samples.
机译：与他们的家长物种相比，杂交寄生虫可能具有增加的传播电位和更高的毒力。因此，杂化检测对于疾病控制至关重要。以前的交叉实验表明，杂交血吸虫卵具有不同的Mor型型。因此，我们将蛋形态与分子标记的性能进行了检测在血吸虫中检测杂交。我们研究了303个末端鸡蛋的形态，源自19个植物植物血吸虫血吸虫血吸虫和塞内加尔畜牧寄生虫血吸虫血症之间的杂交区。蛋尺寸显示出高度的变化，长度为92.4和176.4μm，宽度为35.7和93.0μm。发现任何明显的Mor型斑型，并且所有卵都像不同程度一样，典型的S. haematobium蛋型。然而，同一蛋上的分子分析清楚地显示出两个不同的部分线粒体COX1曲线，即S. Bovis和S. haematobium，并且只有单一的核曲线曲线（S. haematobium）。因此，在这些特定的十字架中，卵形态似乎不是混合血统的良好指标。我们通过讨论在田间样本的高通量筛选背景下检测分子方法的优点和局限性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号