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首页> 外文期刊>Parasitology >MALDI-TOF MS protein profiling for the rapid identification of Chagas disease triatomine vectors and application to the triatomine fauna of French Guiana
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MALDI-TOF MS protein profiling for the rapid identification of Chagas disease triatomine vectors and application to the triatomine fauna of French Guiana

机译：MALDI-TOF MS蛋白质分析，用于快速鉴定CHAGAS疾病Trairiatomine载体和应用于法国圭亚那的Tratiatomine Fauna

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摘要

Triatomines are haematophagous insects involved in the transmission of Trypanosoma cruzi, the aetiological agent of Chagas disease. The vector competence of these arthropods can be highly variable, depending on the species. A precise identification is therefore crucial for the epidemiological surveillance of T. cruzi and the determination of at-risk human populations. To circumvent the difficulties of morphological identification and the lack of comprehensiveness of the GenBank database, we hereby propose an alternative method for triatomine identification. The femurs of the median legs of triatomines from eight different species from French Guiana were subjected to matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) analysis. Method evaluation was performed on fresh specimens and was applied to dry specimens collected between 1991 and 2003. Femur-derived protein extracts provided reproducible spectra within the same species along with significant interspecies heterogeneity. Validation of the study by blind test analysis provided 100% correct identification of the specimens in terms of the species, sex and developmental stage. MALDI-TOF mass spectrometry appears to be a powerful tool for triatomine identification, which is a major step forward in the fight against Chagas disease.
机译：三角瘤是血液流制昆虫，参与触发器瘤Cruzi的传播，Chagas疾病的安全性。根据物种，这些节肢动物的载体能力可以是高度可变的。因此，精确的鉴定对于T.Cruzi的流行病学监测以及风险的危险人群的测定至关重要。为了规避形态识别的困难和Genbank数据库缺乏全面性，我们提出了一种替代方法，用于三角瘤鉴定。来自来自法国圭亚纳的八种不同物种的三角瘤的中位数的股骨受到基质辅助激光解吸/电离飞行时间（MALDI-TOF）分析。方法评价在新鲜标本上进行，并应用于1991年至2003年间收集的干燥标本。股骨衍生的蛋白质提取物在同一物种内提供可再现的光谱，以及显着的间隙异质性。盲试验分析验证研究提供了100％在物种，性和发育阶段的标本正确识别。马尔迪-TOF质谱似乎是Tratiatomine识别的强大工具，这是对抗Chagas疾病的重大一步。

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  • 来源
    《Parasitology》 |2018年第5期|共11页
  • 作者

    MAUREENLAROCHE; JEAN-MICHELBéRENGER; GLADYSGAZELLE; DENISBLANCHET; DIDIERRAOULT; PHILIPPEPAROLA;

  • 作者单位

    Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE) IHU Méditerranée;

    Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE) IHU Méditerranée;

    Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE) IHU Méditerranée;

    Laboratoire Hospitalier Universitaire de Parasitologie et Mycologie Centre Hospitalier A. Rosemon;

    Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE) IHU Méditerranée;

    Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE) IHU Méditerranée;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 寄生虫病;
  • 关键词

    Triatominae; MALDI-TOF; French Guiana; arthropod identification;

    机译：Triatominae;Maldi-Tof;法国圭亚那;节肢动物鉴定;

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