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Advances and challenges in barcoding pathogenic and environmental Leptospira

机译:条形致致病和环境lepterpira的进步和挑战

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摘要

Leptospirosis is a zoonotic bacterial disease of global importance. A large spectrum of asymptomatic animal hosts can carry the infection and contribute to the burden of human disease. Environmental sources of human contamination also point to the importance of a hydrotelluric reservoir. Leptospirosis can be caused by as many as 15 different pathogenic or intermediate Leptospira species. However, classification of these bacteria remains complicated through the use of both serological and genetic classification systems that show poor correlation. With the advent of molecular techniques, DNA-based barcoding offers a conceptual framework that can be used for leptospirosis surveillance as well as source tracking. In this review, we summarize some of the current techniques, highlight significant successes and weaknesses and point to the future opportunities and challenges to successfully establish a widely applicable barcoding scheme for Leptospira.
机译:钩端螺旋体病是一种全球重要性的一种人畜共患病。 大量无症状动物宿主可以携带感染并有助于人类疾病的负担。 人体污染的环境来源也指向水池储层的重要性。 钩端螺旋体病可以由多达15种不同的致病或中间左旋螺杆状物种引起。 然而,通过使用血清学和遗传分类系统,这些细菌的分类仍然是呈现不良相关性的血清学和遗传分类系统。 随着分子技术的出现,基于DNA的条形码提供了一种概念框架,可用于钩螺旋体病监测以及源跟踪。 在这篇综述中,我们总结了一些当前的技术,突出了显着的成功和缺点,并指向未来的机会和挑战,以成功为lepterospira建立广泛适用的条形码方案。

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