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Genetic diversity and phylogeography of the elusive, but epidemiologically important Echinococcus granulosus sensu stricto genotype G3

机译:难以捉摸的遗传多样性和讲学,但流行病学性重要的echinococccus颗粒细胞Sensus starsustogogogo Genotype G3

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摘要

Cystic echinococcosis (CE) is a severe parasitic disease caused by the species complex Echinococcus granulosus sensu lato. Human infections are most commonly associated with E. granulosus sensu stricto (s.s.), comprising genotypes G1 and G3. The objective of the current study was to provide first insight into the genetic diversity and phylogeography of genotype G3. Despite the epidemiological importance of the genotype, it has remained poorly explored due to the ambiguity in the definition of the genotype. However, it was recently demonstrated that long sequences of mitochondrial DNA (mtDNA) provide a reliable method to discriminate G1 and G3 from each other. Therefore, we sequenced near-complete mtDNA of 39 G3 samples, covering most of the known distribution range and host spectra of the genotype. The phylogenetic network revealed high genetic variation within E. granulosus s.s. G3 and while G3 is significantly less prevalent worldwide than G1, the genetic diversity of both of the genotypes is equally high. We also present the results of the Bayesian phylogeographic analysis, which yielded several well-supported diffusion routes of genotype G3 originating from Turkey and Iran, suggesting the Middle East as the origin of the genotype.
机译:囊性超声皮肤病(CE)是由物种复合棘球膜颗粒状胶囊引起的严重寄生疾病。人类感染最常与E.Granulosus Sensu Sticalo(S.)相关的,包括基因型G1和G3。目前研究的目的是提供对基因型G3的遗传多样性和局部摄影的首次见解。尽管基因型的流行病学重要性,但由于基因型定义中的模糊性,它仍然持久探索。然而,最近证明了线粒体DNA(MTDNA)的长序列提供了可靠的方法来彼此区分G1和G3。因此,我们对39g3样品的接近完全MTDNA测序,覆盖了大多数已知的基因型的分布范围和宿主光谱。系统发育网络揭示了大肠杆菌颗粒体内的高遗传变异。 G3虽然G3在全球范围内普遍存存而不是G1,但两种基因型的遗传多样性同样高。我们还介绍了贝叶斯语法分析的结果,它产生了源自土耳其和伊朗的基因型G3的几种良好的扩散途径,表明中东作为基因型的起源。

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