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首页> 外文期刊>Parasitology >Distinct genetic profiles of Leishmania (Viannia), braziliensis associate with clinical variations in cutaneous-leishmaniasis patients from an endemic area in Brazil
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Distinct genetic profiles of Leishmania (Viannia), braziliensis associate with clinical variations in cutaneous-leishmaniasis patients from an endemic area in Brazil

机译：Leishmania（viannia），巴西人的不同遗传谱与巴西地方区域的皮肤 - 利什曼病患者的临床变化

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摘要

American tegumentary leishmaniasis (ATL) samples obtained from the lesions of patients with typical (n = 25, 29%), atypical (n = 60, 69%) or both (n = 2%) clinical manifestations were analysed by multilocus enzyme electrophoresis, hsp70 restriction-fragment length polymorphism (PCR-RFLP), hsp70 sequencing and phylogenetics methods. The hsp70 PCR-RFLP analysis revealed two different profiles whose the most samples differed from those expected for Leishmania braziliensis and the other Leishmania species tested: of 39 samples evaluated, two (5%) had a restriction profile corresponding to L. braziliensis, and 37 (95%) had a restriction profile corresponding to a variant pattern. A 1300-bp hsp70 gene fragment was sequenced to aid in parasite identification and a phylogenetic analysis was performed including 26 consensus sequences from the ATL patient's samples and comparing to other Leishmania and trypanosomatids species. The dendrogram allowed to observe a potential population structure of L. braziliensis complex in the studied region, emphasizing that the majority of clinical samples presented a variant genetic profile. Of interest, the L. braziliensis diversity was associated with different clinical manifestations whose parasites with hsp70 variant profile were associated with atypical lesions. The results may be helpful to improve the diagnosis, treatment and control measures of the ATL in endemic areas.
机译：从典型（n = 25,29％），非典型（n = 60,69％）或两者（n = 2％）临床表现的患者的病变中获得的美国Tegumentary Leishmaniaisis（ATL）样品进行了多点酶电泳， HSP70限制性碎片长度多态性（PCR-RFLP），HSP70测序和系统发育方法。 HSP70 PCR-RFLP分析揭示了两种不同的曲线，其最多的样本与预期的Leishmania Braziliensis和测试的其他LeishMania物种不同：39种样品评估，两（5％）对应于L.Braziliensis的限制性概况，37 （95％）具有对应于变体图案的限制曲线。测序1300-BP HSP70基因片段以帮助寄生虫鉴定，并进行系统发育分析，包括来自ATL患者样品的26个共识序列，并与其他Leishmania和促锥蛋白酰胺酸盐进行比较。使树木图允许观察研究区域L.Bariliensis复合物的潜在人口结构，强调大多数临床样本呈现了变异遗传概况。感兴趣的是，L.Baziliensis多样性与不同的临床表现有关，其寄生虫与HSP70变异型材与非典型病变有关。结果可能有助于改善流行区域ATL的诊断，治疗和控制措施。

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