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首页> 外文期刊>Parasitology >Optimized conditions for the in vitro excystment of Calicophoron daubneyi metacercariae
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Optimized conditions for the in vitro excystment of Calicophoron daubneyi metacercariae

机译：Calicophoron Daubneyi Metacerariae的体外源性优化条件

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摘要

Paramphistomosis, caused by Calicophoron daubneyi, is an emerging infection of ruminants throughout Western Europe. Despite its prevalence, many questions remain regarding the basic biology of this parasite and how it interacts with its host. Consequently, there is a need to develop methods to study C. daubneyi in vitro to improve our understanding of rumen fluke biology. Towards this, we aimed to identify a suitable protocol for in vitro excystment of C. daubneyi metacercariae. Six methods that have been used to excyst metacercariae from a number of trematode species were tested with C. daubneyi metacercariae. Three of these achieved an average of >50% excystment whilst one method, which included an acid-pepsin treatment, incubation in reducing conditions and an alkaline/bile salt solution to activate the larvae, consistently gave >80% excystment. The latter protocol also showed no detrimental effect on the motility of newly excysted juvenile (NEJ) parasites when observed for up to 24 h in RPMI 1640 medium post-excystment. The successful production of C. daubneyi NEJs in vitro is a significant step forward, and will enable the discovery of infective stage-specific parasite antigens and facilitate drug screening trials, to aid the development of much needed diagnostic and therapeutic options for paramphistomosis.
机译：由Calicophoron Daubneyi引起的副血栓药物是在西欧的反刍动物的新兴感染。尽管存在普遍性，但许多问题仍然存在于这种寄生虫的基本生物学以及它如何与其主人互动。因此，需要制定在体外研究C. Daubneyi的方法，以改善我们对瘤胃荧光生物学的理解。为此，我们旨在鉴定C. Daubneyi Metacercariae的体外切除的合适方案。用C. Daubneyi Metacerariae测试了从许多震颤物种中使用的六种方法。其中三种实现平均值> 50％的切除术，而其中一种方法包括酸胃蛋白酶处理，在还原条件和碱性/胆汁盐溶液中孵育以激活幼虫，始终如一地给出> 80％的源。后一种方案也对新激增的少年（NEJ）寄生虫的运动性没有不利影响，当在切除后的RPMI 1640培养基中观察到高达24小时时。在体外成功生产C. Daubneyi Nejs是一个重要的前进，并将能够发现感染性阶段特异性寄生虫抗原，并促进药物筛查试验，帮助开发疗法术治疗的许多诊断和治疗选择。

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