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首页> 外文期刊>Parasitology >Development of a sensitive immunochromatographic kit using fluorescent silica nanoparticles for rapid serodiagnosis of amebiasis
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Development of a sensitive immunochromatographic kit using fluorescent silica nanoparticles for rapid serodiagnosis of amebiasis

机译：使用荧光二氧化硅纳米粒子的敏感免疫色谱试剂盒的开发用于快速血清血症的Amebiasis

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We have previously shown that the C-terminal region of the intermediate subunit of Entamoeba histolytica galactose- and N-acetyl-D-galactosamine-inhibitable lectin (C-Igl) is a useful antigen for serodiagnosis of amebiasis. An immunochromatographic kit was developed using fluorescent silica nanoparticles coated with C-Igl prepared in Escherichia coli. Samples for examination were added to the freeze-dried particles and then applied to the immunochromatographic device, in which a test line on the membrane was also coated with C-Igl. Fluorescent intensity was measured using a hand-held reader. In an evaluation of the kit using a human monoclonal antibody, the minimum amount of C-Igl specific antibody showing positive results was 100 pg. In the evaluation of serum samples with different antibody titers in indirect immunofluorescent antibody tests in the kit, 20 μL of serum was sufficient to obtain positive results at 30 min. Serum samples from symptomatic patients with amebic colitis and amebic liver abscess and those from asymptomatic E. histolytica-cyst carriers showed positive results in the kit. Based on evaluation using sera from healthy controls and patients with other infectious diseases, the sensitivity and specificity of the kit were 100 and 97.6%, respectively. Therefore, we conclude that the newly developed kit is useful for rapid serodiagnosis of amebiasis.
机译：我们之前已经表明，entamoeba组织olytica半乳糖和N-乙酰-d-半乳糖胺抑制凝集素（C-IgL）的中间亚基的C末端区域是AmebiaSis的血清诊断的有用抗原。使用在大肠杆菌中制备的C-IG1涂覆的荧光二氧化硅纳米颗粒开发免疫色谱试剂盒。将用于检查的样品加入到冷冻干燥的颗粒中，然后施加到免疫色谱装置上，其中膜上的测试线也被C-IgL涂覆。使用手持读取器测量荧光强度。在使用人单克隆抗体的试剂盒的评价中，显示阳性结果的最小量的C-IG1特异性抗体为100pg。在评估试剂盒中的间接免疫荧光抗体试验中的不同抗体滴度的血清样品中，20μl血清足以在30分钟的时间内获得阳性结果。来自症状患者的血清样本患有氨基型结肠炎和琥珀酸肝脓肿的患者和无症状的E.组织溶剂 - 囊肿载体的血清样本在试剂盒中显示出阳性结果。基于使用来自健康对照和其他传染病患者的血清的评估，试剂盒的敏感性和特异性分别为100和97.6％。因此，我们得出结论，新开发的试剂盒可用于琥珀类的快速血清诊断。

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