...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Blood coagulation & fibrinolysis: an international journal in haemostasis and thrombosis >Thrombomodulin gene proximal promoter polymorphisms in premature acute coronary syndrome patients in Bahrain
【24h】

Thrombomodulin gene proximal promoter polymorphisms in premature acute coronary syndrome patients in Bahrain

机译：巴林早产急性冠脉综合征患者血栓调节蛋白基因近端启动子多态性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Thrombomodulin is expressed on endothelial cells and monocytes (mTM) where it has an anticoagulant function. Enzymatic cleavage from the cell surface produces soluble thrombomodulin (sTM) in plasma. Abnormal levels of sTM and mutations in the thrombomodulin gene (THBD) are linked to cardiovascular disease. The aim of this study was to investigate THBD proximal promoter mutations and levels of sTM and mTM in men presenting with premature acute coronary syndrome (ACS). This prospective cross-sectional study included 100 adult men with premature ACS (age <55 years) and 60 healthy age-matched controls. Plasma sTM was assayed by ELISA. mTM expression was assessed by flow cytometry with CD141 antibody. The -33 G/A polymorphism was identified by PCR-restriction fragment length polymorphism analysis and the THBD proximal promoter region was sequenced. Significantly lower sTM (P<0.001) and higher mTM (P<0.001) were seen in ACS patients. Heterozygous THBD promoter polymorphisms -33 G/A and -9/-10 GG/AT were found in eight patients and five control individuals. In patients and control individuals, allele frequencies of A were 0.02 and 0.025, and that of AT were 0.025 and 0.017, respectively. There were no significant associations of these polymorphisms with ACS, sTM levels or mTM expression. THBD polymorphisms -33 G/A and -9/-10 GG/AT are present in low frequency in our patient population, and are more frequent in the South Asians as compared to the Arabs. The frequency of -33 G/A is lower, whereas that of -9/-10 GG/AT is higher than that reported in the Orientals. The presence of THBD proximal promoter polymorphisms do not explain variations in levels of sTM and mTM in this patient population. Copyright (C) 2015 Wolters Kluwer Health, Inc. All rights reserved.
机译：血栓调节蛋白在具有抗凝功能的内皮细胞和单核细胞（mTM）上表达。从细胞表面酶切产生血浆中的可溶性血栓调节蛋白（sTM）。 sTM水平异常和血栓调节蛋白基因（THBD）突变与心血管疾病有关。这项研究的目的是调查患有早发性急性冠状动脉综合征（ACS）的男性THBD近端启动子突变以及sTM和mTM水平。这项前瞻性横断面研究包括100例患有ACS的成年男性（年龄<55岁）和60例年龄匹配的健康对照者。通过ELISA测定血浆sTM。通过使用CD141抗体的流式细胞术评估mTM表达。通过PCR-限制性片段长度多态性分析鉴定-33 G / A多态性，并对THBD近端启动子区进行测序。在ACS患者中观察到明显较低的sTM（P <0.001）和较高的mTM（P <0.001）。在8名患者和5名对照个体中发现杂合型THBD启动子多态性-33 G / A和-9 / -10 GG / AT。在患者和对照个体中，A的等位基因频率分别为0.02和0.025，AT的等位基因频率分别为0.025和0.017。这些多态性与ACS，sTM水平或mTM表达没有显着关联。 THBD多态性-33 G / A和-9 / -10 GG / AT在我们的患者人群中以较低的频率出现，与阿拉伯人相比，在南亚人中更常见。 -33 G / A的频率较低，而-9 / -10 GG / AT的频率高于东方人所报告的频率。 THBD近端启动子多态性的存在不能解释该患者人群中sTM和mTM水平的变化。版权所有（C）2015 Wolters Kluwer Health，Inc.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号