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首页> 外文期刊>Pediatric cardiology >Microarray Analysis of Differential Gene Expression Profile Between Human Fetal and Adult Heart
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Microarray Analysis of Differential Gene Expression Profile Between Human Fetal and Adult Heart

机译：人胎儿与成人心脏差异基因表达谱的微阵列分析

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摘要

Although many changes have been discovered during heart maturation, the genetic mechanisms involved in the changes between immature and mature myocardium have only been partially elucidated. Here, gene expression profile changed between the human fetal and adult heart was characterized. A human microarray was applied to define the gene expression signatures of the fetal (13-17 weeks of gestation, n = 4) and adult hearts (30-40 years old, n = 4). Gene ontology analyses, pathway analyses, gene set enrichment analyses, and signal transduction network were performed to predict the function of the differentially expressed genes. Ten mRNAs were confirmed by quantificational real-time polymerase chain reaction. 5547 mRNAs were found to be significantly differentially expressed. "Cell cycle" was the most enriched pathway in the down-regulated genes. EFGR, IGF1R, and ITGB1 play a central role in the regulation of heart development. EGFR, IGF1R, and FGFR2 were the core genes regulating cardiac cell proliferation. The quantificational real-time polymerase chain reaction results were concordant with the microarray data. Our data identified the transcriptional regulation of heart development in the second trimester and the potential regulators that play a prominent role in the regulation of heart development and cardiac cells proliferation.
机译：虽然在心脏成熟期间发现了许多变化，但仅部分阐明了未成熟和成熟心肌之间的变化的遗传机制。这里，特征在于，在人胎儿和成人心脏之间改变基因表达谱。施用人微阵列以定义胎儿的基因表达签名（妊娠13-17周，N = 4）和成人心脏（30-40岁，n = 4）。基因本体分析，途径分析，基因设定富集分析和信号转导网络以预测差异表达基因的功能。通过量化的实时聚合酶链反应确认十个mRNA。发现5547 mRNA被显着表达。 “细胞周期”是下调基因中最富集的途径。 EFGR，IGF1R和ITGB1在心脏发育的调节中起着核心作用。 EGFR，IGF1R和FGFR2是调节心脏细胞增殖的核心基因。量化的实时聚合酶链反应结果与微阵列数据相应。我们的数据确定了第二个三个月的心脏发育的转录调节以及在调节心脏发育和心脏细胞增殖中发挥着突出作用的潜在调节因子。

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  • 来源
    《Pediatric cardiology》 |2017年第4期|共7页
  • 作者

    Geng Zhimin; Wang Jue; Pan Lulu; Li Ming; Zhang Jitai; Cai Xueli; Chu Maoping;

  • 作者单位

    Wenzhou Med Univ Childrens Heart Ctr Affiliated Hosp 2 Wenzhou Zhejiang Peoples R China;

    Wenzhou Med Univ Dept Cardiac Surg Affiliated Hosp 1 Wenzhou Zhejiang Peoples R China;

    Wenzhou Med Univ Childrens Heart Ctr Affiliated Hosp 2 Wenzhou Zhejiang Peoples R China;

    Wenzhou Med Univ Cardiac Regenerat Res Inst Wenzhou Zhejiang Peoples R China;

    Wenzhou Med Univ Cardiac Regenerat Res Inst Wenzhou Zhejiang Peoples R China;

    Wenzhou Med Univ Dept Cardiolgy Affiliated Hosp 1 Wenzhou Zhejiang Peoples R China;

    Wenzhou Med Univ Childrens Heart Ctr Affiliated Hosp 2 Wenzhou Zhejiang Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 儿科学;
  • 关键词

    Microarray; Bioinformatics; Human heart; Fetal; Adult;

    机译：微阵列;生物信息学;人体;胎儿;成人;

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