...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Pedobiologia >Shifts and recovery of soil microbial communities in a 40-year field trial under mineral fertilization
【24h】

Shifts and recovery of soil microbial communities in a 40-year field trial under mineral fertilization

机译：在矿物施肥下40岁田间试验中的土壤微生物群落的转变和恢复

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Inorganic fertilizers have been reported to have effects on both microbial activities and soil microbial community structure. However, the published results are often contradictory. To overcome short-term fluctuations in microbial parameters it is necessary to study their changes over the long term. We investigated the impact of a 40-y inorganic fertilization of a grassland field on selected soil microbial enzymatic activities and on the composition of bacterial and fungal communities, assessed by terminal restriction fragment length polymorphism (T-RFLP). The following fertilizer treatments were compared: C (control, no fertilization), PK (phosphorus and potassium) fertilizer, 80N (lower nitrogen plus PK fertilizer) and 160 N (higher nitrogen plus PK fertilizer). The field trial also included a NF treatment where fertilization with high nitrogen doses was terminated after 20 y, and the subsequent 20 y were under a non-fertilization regime. Except for arylsulfatase, addition of PK was not sufficient intervention to influence enzyme activities. On the contrary, we observed a significant increase in cellobiosidase, phosphomonoesterase, and beta-glucosidase activity in soils fertilized with N, although there was no difference in the enzyme activities between the 80N and 160N treatments. The activities of these enzymes in soils under NF treatment returned to the values of the control soils. Decreased activity of arylsulfatase was detected in treatments with fertilization compared to the control treatment. The shifts in the enzyme activities were accompanied by changes in the composition of whole bacterial and fungal communities, which was also affected by the long-term fertilization. Community composition in fertilized soils clearly differed from the control soils. Contrary to bacteria, 20 y following the cessation of fertilization in the NF treatment was not enough time for complete recovery of the fungal community to that observed in the control treatment. Our findings suggested that soil bacteria are more resilient to chemical fertilization disturbances than fungi.
机译：据报道，无机肥料对微生物活性和土壤微生物群落结构具有影响。但是，公布的结果往往是矛盾的。为了克服微生物参数的短期波动，必须在长期内研究他们的变化。我们研究了由末端限制性片段长度多态性（T-RFLP）评估的末端限制性碎片长度多态性（T-RFLP）对选定的土壤微生物酶活性和细菌和真菌社区组成的影响。比较以下肥料处理：C（对照，无施肥），PK（磷和钾）肥，80N（下氮加PK肥料）和160n（较高的氮加PK肥料）。现场试验还包括NF处理，其中在20 y后终止具有高氮剂量的受精，随后的20 y在不施肥制度下。除芳基硫酸钠外，添加PK是不充分的干预以影响酶活性。相反，我们观察到用n受精的土壤中纤维糖苷酶，磷酸酯酶和β-葡糖苷酶活性的显着增加，尽管80N和160N治疗之间的酶活性没有差异。 NF处理下的土壤中这些酶的活性恢复到对照土壤的值。与对照处理相比，在受精处理中检测到芳基硫酸钠酶的活性降低。酶活性的转变伴随着整个细菌和真菌社区组成的变化，这也受长期施肥的影响。受精土壤中的社区组成明显不同于对照土壤。与细菌相反，在NF治疗中施肥的停止后，20 y是不够的时间来完全恢复真菌群落，在对照治疗中观察到。我们的研究结果表明土壤细菌比真菌更具弹性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号