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首页> 外文期刊>Pediatric allergy and immunology: official publication of the European Society of Pediatric Allergy and Immunology >Detection of genuine grass pollen sensitization in children by skin testing with a recombinant grass pollen hybrid
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Detection of genuine grass pollen sensitization in children by skin testing with a recombinant grass pollen hybrid

机译：用重组草花粉杂种皮肤测试检测儿童真正草花粉致敏

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Background Skin testing represents a commonly used first diagnostic method in clinical practice, but allergen extracts may vary in composition and often contain cross-reactive allergens and therefore do not always allow the precise identification of the sensitizing allergen source. Our aim was to investigate the suitability of a single recombinant hybrid molecule, consisting of the four major timothy grass pollen allergens (Phl p 1, Phl p 2, Phl p 5, and Phl p 6) for in vivo diagnosis of genuine grass pollen allergy in children suffering from pollinosis. Methods Sixty-four children aged from 6 to 17 years with a positive skin reaction and/or specific IgE to grass pollen extract and respiratory symptoms of pollinosis as well as 9 control children with allergy to other allergen sources were studied. SPT was performed with the recombinant hybrid, the four recombinant timothy grass pollen allergens, and grass pollen extract. Specific IgE reactivity to 176 micro-arrayed allergen molecules was determined using ImmunoCAP ISAC technology. IgE reactivity to the hybrid was detected by non-denaturing RAST-based dot blot assay. Results Genuine grass pollen sensitization was confirmed in 94% of the children with positive SPT to grass pollen extract by SPT and IgE reactivity to the hybrid. The four hybrid-negative children showed IgE reactivity to cross-reactive allergens such as Phl p 4, Phl p 11, and Phl p 12 and had also sensitizations to pollen allergens from unrelated plants. Conclusions The recombinant hybrid molecule represents a useful tool for in vivo diagnosis of genuine grass pollen sensitization.
机译：背景技术皮肤测试代表临床实践中常用的第一诊断方法，但过敏原提取物可以在组成中变化并且通常含有交叉反应性过敏原，因此并不总是允许敏化过敏原源的精确鉴定。我们的目的是探讨单一重组杂交分子的适用性，由四个主要的蒂莫西草花粉过敏原（PHL P 1，PHL P 2，PHL P 5和PHL P 6）组成，用于诊断正品草花粉过敏的诊断在患有花粉的儿童中。方法采用阳性皮肤反应和阳性皮肤反应和/或特异性IgE对草花粉提取物和呼吸道症状以及其他过敏原源过敏的呼吸道症状的六十四名儿童。 SPT用重组杂交，四个重组暗症草花粉过敏原，草花粉提取物进行。使用Immuncocap ISAC技术测定特异性IgE反应性至176个微串过敏原分子。通过非变性的基于RAST基点印迹测定检测对杂种的反应性。结果在94％的儿童中确认了Gress Pollen提取物的94％的儿童对杂种的阳性，结果确认了草花粉致敏。四种杂化阴性儿童向Ige反应性过敏原如PHL P 4，PHL P 11和PHL P 12表示IgE反应性，并且对来自无关植物的花粉过敏原也具有敏感性。结论重组杂交分子代表了用于真正草花粉致敏的体内诊断的有用工具。

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