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首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >Development of a Novel One-Step Automated Rapid in situ Hybridization for Anaplastic Lymphoma Kinase Rearrangement Using Non-Contact Alternating-Current Electric-Field Mixing
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Development of a Novel One-Step Automated Rapid in situ Hybridization for Anaplastic Lymphoma Kinase Rearrangement Using Non-Contact Alternating-Current Electric-Field Mixing

机译：使用非接触交流电场混合开发一种新型单步自动化自动杂交，用于使用非接触交流电 - 电场混合进行制备淋巴瘤激酶重排

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摘要

Echinoderm microtubule-associated protein-like 4 and anaplastic lymphoma kinase (ALK) fusion gene rearrangement is a key driver mutation in non-small cell lung cancer (NSCLC). Although Break-Apart ALK fluorescence in situ hybridization (FISH) is a reliable diagnostic method for detecting ALK gene rearrangement, it is also costly and time-consuming to use as a routine screening test. Our aim was to evaluate the clinical utility of a novel one-step, automated, rapid FISH (Auto-RaFISH) method developed to facilitate hybridization. This method takes advantage of the non-contact mixing effect of an alternating-current electric field. Ten representative specimens from 85 patients diagnosed at multiple centers with primary lung cancer with identified ALK-FISH status were collected. The specimens were all tested using FISH, RaFISH, and Auto-RaFISH. With both RaFISH protocols, the ALK test was completed within 4.5 h, as compared to the 20 h needed for the standard protocol. We found 100% agreement between the standard FISH, RaFISH, and new Auto-RaFISH based on the ALK status, and all methods stained equally well. These findings suggest that Auto-RaFISH could potentially serve as an automated clinical tool for prompt determination of ALK status in NSCLC.
机译：Echinoderm微管相关的蛋白质样4和促进淋巴瘤激酶（ALK）融合基因重排是非小细胞肺癌（NSCLC）的关键驱动突变。尽管原位杂交（鱼类）破裂的ALK荧光是用于检测ALK基因重排的可靠诊断方法，但用作常规筛查测试也是昂贵且耗时的。我们的目标是评估开发的新型一步，自动化，快速鱼（自动rafish）方法的临床效用，以促进杂交。该方法利用交流电场的非接触式混合效果。收集了来自85名患者的十个代表性标本，诊断为具有鉴定的ALK鱼状态的原发性肺癌。这些标本都使用鱼，rafish和自动rafish进行测试。通过Rafish方案，ALK测试在4.5小时内完成，与标准方案所需的20小时相比。我们在标准鱼，Rafish和新的自动rafish之间找到了100％的协议，基于ALK状态，所有方法都同样良好染色。这些发现表明，自动rafish可能是潜在的作为迅速确定NSCLC中ALK状态的自动临床工具。

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