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首页> 外文期刊>Pest Management Science >Transcription factor FTZ-F1 and cis-acting elements mediate expression of CYP6BG1 conferring resistance to chlorantraniliprole in Plutella xylostella
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Transcription factor FTZ-F1 and cis-acting elements mediate expression of CYP6BG1 conferring resistance to chlorantraniliprole in Plutella xylostella

机译：转录因子FTZ-F1和顺式作用元件介导CYP6BG1的表达赋予Plutella Xylostella的氯苯吡啶潜力

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BACKGROUND Cytochrome P450-mediated detoxification plays an important role in the development of insecticide resistance. Previous studies have demonstrated that overexpression of CYP6BG1 was responsible for permethrin resistance in Plutella xylostella, and our experiments also showed that upregulation of this gene is associated with chlorantraniliprole resistance in P. xylostella. However, the transcriptional regulation involved in the expression of CYP6BG1 remains unknown. To further investigate the regulation of CYP6BG1 expression, the promoters of this gene were cloned and analyzed from one susceptible and four different resistant populations of P. xylostella. RESULTS First, the promoter region of P. xylostella CYP6BG1 was compared in five populations, and three types of 5 '-flanking region were found. Second, the region between -562 and +49 of CYP6BG1 in a field population (TH) of P. xylostella showed the highest promoter activity and could be induced by chlorantraniliprole. Third, the transcriptional factor FTZ-F1, which is an orphan nuclear receptor and binds to the fushi tarazu (ftz) gene, was predicted by the online software Alggen and Jaspar. It was proved to regulate the expression of CYP6BG1 by RNAi. The expression levels of FTZ-F1 and CYP6BG1 could be induced by chlorantraniliprole and were significantly higher in the resistant populations. CONCLUSIONS These data give a better understanding of the transcriptional regulation of an important insecticide detoxification enzyme gene, and therefore will help in understanding the molecular mechanisms of insecticide resistance in P. xylostella. (c) 2018 Society of Chemical Industry
机译：背景技术细胞色素P450介导的排毒在杀虫剂抗性的发展中起重要作用。以前的研究表明，CYP6BG1的过度表达对Plutella Xylostella的耐受素抗性负责，并且我们的实验还表明该基因的上调与氯化菌菌的氯苯胆抗抗性有关。然而，CYP6BG1表达中涉及的转录调节仍然未知。为了进一步研究CYP6BG1表达的调节，克隆了该基因的启动子并从一种易感和四种不同的P. Xylostella抗性群体分析。结果首先，在五种群体中比较了P.Xylostella Cyp6BG1的启动子区，发现了三种类型的5'粒状区域。其次，P.Xylostella的野外群体（Th）的CYP6BG1之间的-562和+49之间的区域显示出最高的启动子活性，并且可以通过氯苯胆胶诱导。第三，通过在线软件Alggen和Jaspar预测，作为孤儿核受体并结合Fushi Tarazu（FTZ）基因的转录因子FTZ-F1。事实证明，通过RNAi调节CYP6BG1的表达。 COLALANTRANILIPROLE可以诱导FTZ-F1和CYP6BG1的表达水平，抗性群体显着较高。结论这些数据更好地了解重要的杀虫剂排毒酶基因的转录调节，因此有助于了解杀虫剂抗性的杀虫剂抗性的分子机制。 （c）2018化学工业协会

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