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首页> 外文期刊>Pest Management Science >Transcriptomic analysis of interactions between Hyphantria cunea larvae and nucleopolyhedrovirus
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Transcriptomic analysis of interactions between Hyphantria cunea larvae and nucleopolyhedrovirus

机译：菌根丘亚氏幼虫与核多肽之间的相互作用转录组分析

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BACKGROUND Hyphantria cunea is a destructive forest pest. To utilise H. cunea nucleopolyhedrovirus (HcNPV) for biological control, understanding insect-virus interactions is essential. RESULTS Four cDNA libraries were constructed from H. cunea larvae (two HcNPV-infected and two uninfected groups) and 76 004 expressed genes were obtained by next-generation sequencing. Compared with controls, 272 differentially expressed genes (DEGs) were identified in infected groups, including 162 up-regulated and 110 down-regulated genes. Transcription levels of 20 random DEGs were consistent with the Solexa expression profiles obtained by quantitative real-time PCR. DEGs associated with innate immunity were grouped into several categories, including pattern recognition proteins, heat-shock proteins, UDP-glycosyltransferases, cytochrome P450s, antimicrobial peptides and hormonal signalling proteins. Interestingly, up-regulated host genes included farnesoic acid O-methyltransferase, two juvenile hormone (JH) binding proteins, and a circadian clock-controlled protein related to JH regulation. Pathway enrichment analysis indicates that mitogen-activated protein kinase (MAPK) signalling pathways, key candidate genes and important biological pathways may be associated with molecular modification in H. cunea larvae in response to virus stress. CONCLUSION These findings provide insight for future research on the molecular mechanisms of HcNPV invasion and anti-HcNPV mechanisms in H. cunea. A better understanding of gene regulation following HcNPV invasion could help to develop the virus as a bio-insecticide. (c) 2018 Society of Chemical Industry
机译：背景Hyphantria Cunea是一种破坏性的森林害虫。利用H.Cunea核心过滤的生物控制，了解昆虫病毒相互作用至关重要。结果通过下一代测序从下一代测序中由H. Cunea幼虫构建四个cDNA文库（两个HCNPV感染和两个未感染的基团）和76 004表达基因。与对照相比，在感染的基团中鉴定了272个差异表达基因（DEGS），其中包括162个上调和110个下调基因。转录水平为20次随机液的含量与通过定量实时PCR获得的SOLEX表达谱一致。将与先天免疫相关的DEG分为几个类别，包括模式识别蛋白，热休克蛋白，UDP-糖基转移酶，细胞色素P450s，抗微生物肽和激素信号传导蛋白。有趣的是，上调的宿主基因包括法牛酸O-甲基转移酶，两种幼年激素（JH）结合蛋白，以及与JH调节有关的昼夜钟控制蛋白。途径富集分析表明丝裂原激活的蛋白激酶（MAPK）信号传导途径，关键候选基因和重要的生物途径可能与H. Cunea幼虫的分子修饰相关，响应于病毒胁迫。结论这些调查结果为未来的HCNPV侵袭和抗HCNPV机制的未来研究提供了洞察力。 HCNPV侵袭后对基因调节的更好理解可以有助于将病毒作为生物杀虫剂发展。 （c）2018化学工业协会

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