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首页> 外文期刊>Pest Management Science >Identification of a novel cytochrome P450 CYP3356A1 linked with insecticide detoxification in Bradysia odoriphaga
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Identification of a novel cytochrome P450 CYP3356A1 linked with insecticide detoxification in Bradysia odoriphaga

机译：鉴定Bradysia Odoriphaga杀虫剂排毒的新细胞色素P450 CYP3356A1

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摘要

BACKGROUND Cytochrome P450 monooxygenases play an important role in the metabolic detoxification of insecticides in insect pests. However, little is known about the role of a specific P450 gene and its responses to insecticide exposure in Bradysia odoriphaga, a major pest in Chinese chive production. RESULTS In this study, a novel P450 gene, CYP3356A1, was cloned from Bradysia odoriphaga. The full-length cDNA sequence of CYP3356A1 is 2153 bp and its open reading frame (ORF) encodes 508 amino acids. Quantitative real time PCR(qRT-PCR) analyses in different tissues showed that CYP3356A1 expression was the highest in the Malpighian tubule. Moreover, among the different developmental stages of the insect, the highest expression of CYP3356A1 was found in fourth-instar larvae. Expression of CYP3356A1 was upregulated by treatment with imidacloprid, thiamethoxam, and beta-cypermethrin at median lethal concentrations (LC50). RNA interference (RNAi)-mediated silencing of CYP3356A1 significantly increased mortality by 36.90%, 25.17%, and 36.73 when fourth-instar B. odoriphaga larvae were exposed to imidacloprid, thiamethoxam, and beta-cypermethrin, respectively, at the LC50 dose. CONCLUSION These results demonstrate that CYP3356A1 is related to the detoxification of imidacloprid, thiamethoxam, and beta-cypermethrin in B. odoriphaga. Moreover, the study also increased our understanding of the molecular mechanisms of insecticide detoxification in this pest insect. (c) 2018 Society of Chemical Industry
机译：背景技术细胞色素P450单氧基酶在昆虫害虫中的杀虫剂的代谢解毒中起重要作用。然而，关于特定P450基因的作用及其对致命性杀虫剂暴露的作用的少数人少知之甚少，这是中国韭菜生产中的一个主要害虫。结果在该研究中，来自Bradysia Odoriphaga的新型P450基因CYP3356A1。 CYP3356A1的全长cDNA序列是2153bp，其开放阅读框（ORF）编码508个氨基酸。不同组织中的定量实时PCR（QRT-PCR）分析表明，CYP3356A1表达在Malpighian小管中是最高的。此外，在昆虫的不同发育阶段中，CYP3356A1的最高表达在第四龄幼虫中发现。通过用吡虫啉，硫醚，硫昔氯吡啶和β-吡喃芬菊酯治疗中值致命浓度（LC50）来上调CYP356A1的表达。在LC50剂量分别在LC50剂量分别在LC50剂量下，CYP3356A1的RNA干扰（RNAi）介绍的沉默显着提高了36.90％，25.17％和36.73。结论这些结果表明，CYP3356A1与吡虫啉，噻虫草醛和Beta-Cipetmethrin的解毒有关，B. Odoripha。此外，该研究还提高了我们对这种害虫昆虫中杀虫剂排毒的分子机制的理解。 （c）2018化学工业协会

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