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首页> 外文期刊>Phytopathology >Transgenic Expression of EFR and Bs2 Genes for Field Management of Bacterial Wilt and Bacterial Spot of Tomato
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Transgenic Expression of EFR and Bs2 Genes for Field Management of Bacterial Wilt and Bacterial Spot of Tomato

机译：EFR和BS2基因的转基因表达，用于番茄细菌枯萎病和细菌斑的田间管理

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摘要

Field trials were conducted at two locations in Florida to evaluate transgenic tomato expressing the ELONGATION FACTOR TU RECEPTOR (EFR) gene from Arabidopsis thaliana, the Bs2 gene from pepper, or both Bs2 and EFR (Bs2/EFR) for managing bacterial wilt caused by Ralstonia solanacearum and bacterial spot caused by Xanthomonas perforans. Expression of EFR or Bs2/EFR in the susceptible genotype Fla. 8000 significantly reduced bacterial wilt incidence (50 to 100%) and increased total yield (57 to 114%) relative to lines expressing only Bs2 or the nontransformed Fla. 8000 control, although the marketable yield was not significantly affected. Following harvest, surviving symptomatic and non-symptomatic plants were assessed for colonization by R. solanacearum. There were no significant differences in the population at the lower stem. Interestingly, in the middle stem, no bacteria could be recovered from EFR or Bs2/EFR lines but viable bacterial populations were recovered from Bs2 and nontransformed control lines at 10(2) to 10(5) CFU/g of stem tissue. In growth-chamber experiments, the EFR transgenic tomato lines were found to be effective against seven different R. solanacearum strains isolated from the southeastern United States, indicating utility across the southeastern United States. In all of the bacterial spot trials, EFR and Bs2/EFR lines had significantly reduced disease severity (22 to 98%) compared with the Fla. 8000 control. The marketable and total yield of Bs2/EFR were significantly higher (43 to 170%) than Fla. 8000 control in three of four field trials. These results demonstrate for the first time the potential of using the EFR gene for field management of bacterial wilt and bacterial spot diseases of tomato.
机译：在佛罗里达州的两个位置进行现场试验，以评估从拟南芥，来自胡椒的BS2基因的伸长因子Tu受体（EFR）基因的转基因番茄，或用于管理由Ralstonia引起的细菌枯萎病的BS2和EFR（BS2 / EFR）。 Xanthomonas穿孔血症引起的Solanacearum和细菌斑点。 EFR或BS2 / EFR在易感基因型FLA中的表达。8000显着降低的细菌枯萎发生率（50至100％），相对于仅表达BS2的线或非转化的FLA增加，增加总率（57至114％）。8000控制，虽然市场营销产量没有受到显着影响。收获后，通过R.Solanacearum评估症状和非症状植物的存活。下茎的人口没有显着差异。有趣的是，在中间茎中，不能从EFR或BS2 / EFR线中回收细菌，但在10（2）至10（5）CFU / g的茎组织中，从BS2和非转化的对照线中回收活细菌群。在生长室实验中，发现EFR转基因番茄素源性针对从美国东南部分离的七种不同的R. Solanacearum菌株有效，表明在美国东南部的效用。在所有细菌现场试验中，与FLA相比，EFR和BS2 / EFR线显着降低了疾病严重程度（22至98％）。8000控制。 BS2 / EFR的可营销和总产量明显高于（43〜170％），而不是FLA。在四个现场试验中有三种控制。这些结果首次证明了使用EFR基因进行番茄细菌枯萎和细菌斑疾病的田间管理的潜力。

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  • 来源
    《Phytopathology》 |2018年第12期|共10页
  • 作者

    Kunwar Sanju; Iriarte Fanny; Fan Qiurong; da Silva Eduardo Evaristo; Ritchie Laura; Nghi Song Nguyen; Freeman Joshua H.; Stall Robert E.; Jones Jeffrey B.; Minsavage Gerald V.; Colee James; Scott Jay W.; Vallad Gary E.; Zipfel Cyril; Horvath Diana; Westwood Jack; Hutton Samuel F.; Paret Mathews L.;

  • 作者单位

    Univ Florida Inst Food &

    Agr Sci Dept Plant Pathol Gainesville FL 32611 USA;

    Univ Florida Inst Food &

    Agr Sci North Florida Res &

    Educ Ctr Quincy FL 32351 USA;

    Univ Florida Inst Food &

    Agr Sci Dept Plant Pathol Gainesville FL 32611 USA;

    Univ Florida Inst Food &

    Agr Sci North Florida Res &

    Educ Ctr Quincy FL 32351 USA;

    Univ Florida Inst Food &

    Agr Sci North Florida Res &

    Educ Ctr Quincy FL 32351 USA;

    Univ Florida Inst Food &

    Agr Sci North Florida Res &

    Educ Ctr Quincy FL 32351 USA;

    Univ Florida Inst Food &

    Agr Sci North Florida Res &

    Educ Ctr Quincy FL 32351 USA;

    Univ Florida Inst Food &

    Agr Sci Dept Plant Pathol Gainesville FL 32611 USA;

    Univ Florida Inst Food &

    Agr Sci Dept Plant Pathol Gainesville FL 32611 USA;

    Univ Florida Inst Food &

    Agr Sci Dept Plant Pathol Gainesville FL 32611 USA;

    Univ Florida Inst Food &

    Agr Sci Div Stat Gainesville FL 32611 USA;

    Univ Florida Inst Food &

    Agr Sci Dept Hort Sci Gainesville FL 32611 USA;

    Univ Florida Inst Food &

    Agr Sci Dept Plant Pathol Gainesville FL 32611 USA;

    Sainsbury Lab Norwich Res Pk Norwich NR4 7UH Norfolk England;

    Two Blades Fdn Evanston IL 60201 USA;

    Two Blades Fdn Evanston IL 60201 USA;

    Univ Florida Inst Food &

    Agr Sci Dept Hort Sci Gainesville FL 32611 USA;

    Univ Florida Inst Food &

    Agr Sci Dept Plant Pathol Gainesville FL 32611 USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物病理学;
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