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首页> 外文期刊>Physiological and Molecular Plant Pathology >DNA-based electrochemical nanobiosensor for the detection of Phytophthora palmivora (Butler) Butler, causing black pod rot in cacao (Theobroma cacao L.) pods
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DNA-based electrochemical nanobiosensor for the detection of Phytophthora palmivora (Butler) Butler, causing black pod rot in cacao (Theobroma cacao L.) pods

机译：基于DNA的电化学纳米极细管传感器，用于检测植物肺胰岛素（BUTLER）管家，导致恶石瓜（CacroomaCaO1）豆荚

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摘要

A nanobiosensor was developed for the electrochemical detection of Phytophthora palmivora, a notorious pathogen of cacao causing severe crop loss. Sandwich hybrids between two oligonucleotide probes and the genomic DNA of P. palmivora were prepared and electrochemically detected. The oligonucleotide probes were designed based on the ITS sequence of the P. palmivora field isolates. The detection of sandwich hybrids with P. palmivora genomic DNA and the selectivity of the nanobiosensor towards it compared to other cacao-associated fungal pathogens were demonstrated. The detection limit was determined to be at 0.30 ng DNA mu L-1. Detection of P. palmivora DNA on cacao samples was also demonstrated. The result of the preliminary analysis on cacao pod samples shows the potential of the developed nanobiosensor for reliable and more cost-effective analysis of field samples.
机译：开发了一种纳米源传感器，用于植物的电化学检测植物的棕榈酰虫，是可可病原体的臭名昭着的病原体导致严重的作物丧失。 制备两种寡核苷酸探针与P. palmivora的基因组DNA之间的夹层杂种和电化学检测。 基于其P. palmivora野外分离株的序列设计了寡核苷酸探针。 对与其他可可病原体相比，用P. palmivora基因组DNA检测与P. palmivora基因组DNA的检测和纳米极细管传感器的选择性。 检测限定为0.30ng DNA m l-1。 还证明了对可可样品上的P. palmivora DNA的检测。 CACAO POD样品初步分析的结果表明，发育的纳米极高传感器的潜力可用于对场样品的可靠和更具成本效益分析。

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